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Immunochromatographic assay for T-2 toxin based on luminescent quantum dot beads

机译：基于发光量子点珠的T-2毒素的免疫层析测定

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摘要

A quantum dot bead based immunochromatographic assay (QB-ICA) system was established for T-2 toxin (T-2), which widely occurs in agriculture and could be used as a potential biological warfare agent. After optimization, the dynamic linear detection range of T-2 calculated from a calibration curve was from 0.12 to 0.67 ng mL ~(?1) and the limit of detection (LOD) was 0.08 ng mL ~(?1) , which is lower than those of the ICA based on colloidal gold nanoparticles or a fluorescent material or an antibody-based biochip in other reports. The performance and practicability of the established ICA system were validated with a commercial ELISA kit and the two methods were comparable. The proposed QB-ICA for T-2 could be an alternative for rapid, sensitive, and quantitative on-site detection of this toxin in biosafety monitoring in agriculture and for susceptibility testing of the potential release of this biological warfare agent.
机译：为T-2毒素（T-2）建立了量子点胎珠的免疫粒状测定（QB-ICA）系统，其在农业中广泛发生，可用作潜在的生物战争剂。优化后，从校准曲线计算的T-2的动态线性检测范围为0.12至0.67 ng ml〜（Δ1），检测极限（LOD）为0.08 ng ml〜（Δ1），这是较低的而不是基于胶体金纳米颗粒或荧光材料或其他报告中的抗体的生物芯片的ICA的那些。已建立的ICA系统的性能和实用性用商业ELISA试剂盒验证，两种方法是可比的。对于T-2的提议的QB-ICA可以是替代于农业生物安全监测中这种毒素的快速，敏感和定量的现场检测的替代方案，以及这种生物战争潜在释放的易感性检测。

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