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首页> 外文期刊>RSC Advances >Simultaneous quantification of five biomarkers in ethanolic extract of Cassia occidentalis Linn. stem using liquid chromatography tandem mass spectrometry: application to its pharmacokinetic studies
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Simultaneous quantification of five biomarkers in ethanolic extract of Cassia occidentalis Linn. stem using liquid chromatography tandem mass spectrometry: application to its pharmacokinetic studies

机译：Cassia Occidentalis LINN乙醇提取物同时定量五种生物标志物。茎使用液相色谱串联质谱：应用于其药代动力学研究

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Cassia occidentalis L. stem extract is used as a purgative, febrifuge, and diuretic, and in the treatment of flu, fever, fracture and bone diseases. Pharmacological studies prove the osteogenic and antiresorptive effects of Cassia occidentalis L. ethanolic extract (COEE), which may be due to apigenin, apigenin-6- C -glucopyranoside, luteolin, 3′,4′,7-trihydroxyflavone and emodin. The objectives of this study was to develop a selective and sensitive LC-MS/MS method and validate for the simultaneous determination of the above five biomarkers in rat plasma after oral administration of COEE at a dose of 500 mg kg ~(?1) . The analytes were separated on a Phenomenex Luna C18 column (4.6 × 150 mm, 3.0 μm) with an isocratic mobile phase consisting of methanol-10 mM ammonium acetate buffer (95?:?05, v/v). Run time was for 5.5 min with LLOQ of 1 ng mL ~(?1) for all the analytes. The mass spectrometer was operating in negative ionization mode for quantification of the analytes. The calibration curves were linear ( r ~(2) > 0.99) for all the analytes. The intra- and inter-day precisions were less than 8.17% and the relative error was between ?8.57% and 7.28%. Analytes were rapidly absorbed in the oral pharmacokinetic study. The biomarkers were stable in simulated gastric and intestinal fluids but underwent metabolism in rat liver microsomes. This is the first report on in vivo oral pharmacokinetics and in vitro stability studies of osteogenic compounds present in COEE. These results will be helpful for further understanding of pharmacodynamic behaviour of COEE and the bioanalytical method will be useful for further preclinical/clinical trials.
机译：Cassia Occidentalis L.茎提取物用作泻药，FeBrifuge和利尿，以及治疗流感，发热，骨折和骨病。药理研究证明了Cassia occidentalis L.乙醇提取物（Coee）的成骨和反射效果，这可能是由于Apigenin，Apigenin-6-戊酰基吡喃糖苷，叶氏素，3'，4'，7-三羟基氟和大黄素。本研究的目的是开发一种选择性和敏感的LC-MS / MS方法，并验证在口服施用大鼠血浆中以500mg kg〜（α1）的剂量后的大鼠血浆中上述五个生物标志物。将分析物分析在现象Luna C18柱（4.6×150mm，3.0μm）上，其具有由甲醇-10mM醋酸铵缓冲液组成的等级流动相（95Ω：05，v / v）。运行时间为5.5分钟，LLOQ为所有分析物提供1 ng ml〜（？1）。质谱仪以负电离模式运行以定量分析物。所有分析物的校准曲线是线性的（R〜（2）> 0.99）。内部和日内的诊断小于8.17％，相对误差在Δ8.8.57％和7.28％之间。在口腔药代动力学研究中迅速吸收分析物。生物标志物在模拟胃和肠液中稳定，但在大鼠肝微粒体中接受了代谢。这是CoEE中存在的骨质原子油药代动力学和体外稳定性研究的第一报告。这些结果将有助于进一步了解Coee的药效表法，生物分析方法对进一步的临床前/临床试验有用。

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