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首页> 外文期刊>RSC Advances >In vitro and in vivo biocompatibility and inflammation response of methacrylated and maleated hyaluronic acid for wound healing
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In vitro and in vivo biocompatibility and inflammation response of methacrylated and maleated hyaluronic acid for wound healing

机译：体外和体内生物相容性和甲基丙烯酸丙烯酸溶液的炎症反应，用于伤口愈合

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Over the past few years, different in vitro and in vivo studies have been highlighting the great potentiality of hyaluronic acid (HA) as a biomaterial in wound healing treatment thanks to its good capability to induce mesenchymal and epithelial cell growth and differentiation, angiogenesis, and collagen deposition. However, the need to improve its mechanical properties as well as its residence time has led scientists to study new functionalization strategies. In this work, chemically modified HA-based hydrogels were obtained by methacrylic and maleic functionalization. Methacrylated (MEHA) and maleated HA (MAHA) hydrogels have shown important physico-chemical properties. The present study provides a deeper insight into the biocompatibility of both synthesized materials and their effects on tissue inflammation using in vitro and in vivo models. To this aim, different cell lines involved in wound healing, human dermal fibroblasts, human adipose-derived stem cells and human umbilical vein endothelial cells, were seeded on MEHA and MAHA hydrogels. Furthermore, an inflammation study was carried out on a murine macrophage cell line to assess the effects of both hydrogels on inflammatory and anti-inflammatory interleukin production. The results showed that both MAHA and MEHA supported cell proliferation with anti-inflammation ability as highlighted by the increased levels of IL-10 (57.92 ± 9.87 pg mL ~(?1) and 68.08 ± 13.94 pg mL ~(?1) , for MEHA and MAHA, respectively). To investigate the inflammatory response at tissue/implant interfaces, an in vivo study was also performed by subcutaneous implantation of the materials in BALB/c mice for up to 28 days. In these analyses, no significant chronic inflammation reaction was demonstrated in either MEHA or MAHA in the long-term implantation.
机译：在过去的几年里，不同的体外和体内研究已经突出了透明质酸（HA）作为伤口愈合治疗的生物材料的巨大潜力，因此由于其诱导间充质和上皮细胞生长和分化，血管生成，和分化，血管生成和分化胶原沉积。然而，需要改善其机械性能以及其停留时间，LED科学家研究了新的功能化策略。在这项工作中，通过甲基丙烯酸和马来官能化获得化学改性的HA基水凝胶。甲基丙烯酸酯（MEHA）和MALEALED HA（MAHA）水凝胶显示出重要的物理化学性质。本研究提供了深入了解合成材料的生物相容性及其在体外和体内模型中对组织炎症的影响。为此目的，涉及伤口愈合，人的皮肤成纤维细胞，人脂肪衍生的干细胞和人脐静脉内皮细胞的不同细胞系在Meha和Maha水凝胶上播种。此外，在鼠巨噬细胞系上进行炎症研究，以评估Hard凝胶对炎症和抗炎白细胞介素产生的影响。结果表明，马哈和MEHA都支持抗炎能力，突出的IL-10水平突出显示（57.92±9.87 pg ml〜（α1）和68.08±13.94 pg ml〜（？1），适用于Meha和Maha分别）。为了探讨组织/植入界面的炎症反应，还通过皮下植入BALB / C小鼠中的材料进行高达28天进行的体内研究。在这些分析中，在长期植入中没有在Meha或Maha中证明了显着的慢性炎症反应。

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