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首页> 外文期刊>RSC Advances >In vitroandin vivobiocompatibility and inflammation response of methacrylated and maleated hyaluronic acid for wound healing
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In vitroandin vivobiocompatibility and inflammation response of methacrylated and maleated hyaluronic acid for wound healing

机译：在vitroandin的体长和甲基丙烯酸丙烯酸盐和透明质酸的炎症反应进行伤口愈合

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Over the past few years, differentin vitroandin vivostudies have been highlighting the great potentiality of hyaluronic acid (HA) as a biomaterial in wound healing treatment thanks to its good capability to induce mesenchymal and epithelial cell growth and differentiation, angiogenesis, and collagen deposition. However, the need to improve its mechanical properties as well as its residence time has led scientists to study new functionalization strategies. In this work, chemically modified HA-based hydrogels were obtained by methacrylic and maleic functionalization. Methacrylated (MEHA) and maleated HA (MAHA) hydrogels have shown important physico-chemical properties. The present study provides a deeper insight into the biocompatibility of both synthesized materials and their effects on tissue inflammation usingin vitroandin vivomodels. To this aim, different cell lines involved in wound healing, human dermal fibroblasts, human adipose-derived stem cells and human umbilical vein endothelial cells, were seeded on MEHA and MAHA hydrogels. Furthermore, an inflammation study was carried out on a murine macrophage cell line to assess the effects of both hydrogels on inflammatory and anti-inflammatory interleukin production. The results showed that both MAHA and MEHA supported cell proliferation with anti-inflammation ability as highlighted by the increased levels of IL-10 (57.92 +/- 9.87 pg mL(-1)and 68.08 +/- 13.94 pg mL(-1), for MEHA and MAHA, respectively). To investigate the inflammatory response at tissue/implant interfaces, anin vivostudy was also performed by subcutaneous implantation of the materials in BALB/c mice for up to 28 days. In these analyses, no significant chronic inflammation reaction was demonstrated in either MEHA or MAHA in the long-term implantation.
机译：在过去的几年里，不同的vitroandin Vivostudies一直突出透明质酸（HA）作为伤口愈合治疗中的生物材料的巨大潜力，因此由于其诱导间充质和上皮细胞生长和分化，血管生成和胶原沉积的良好能力。然而，需要改善其机械性能以及其停留时间，LED科学家研究了新的功能化策略。在这项工作中，通过甲基丙烯酸和马来官能化获得化学改性的HA基水凝胶。甲基丙烯酸酯（MEHA）和MALEALED HA（MAHA）水凝胶显示出重要的物理化学性质。本研究提供了深入了解合成材料的生物相容性及其对培体Vivomodels的组织炎症的影响。为此目的，涉及伤口愈合，人的皮肤成纤维细胞，人脂肪衍生的干细胞和人脐静脉内皮细胞的不同细胞系在Meha和Maha水凝胶上播种。此外，在鼠巨噬细胞系上进行炎症研究，以评估Hard凝胶对炎症和抗炎白细胞介素产生的影响。结果表明，马哈和Meha都支持抗炎能力，突出的IL-10水平突出（57.92 +/- 9.87 pg ml（-1）和68.08 +/- 13.94 pg ml（-1） ，分别为Meha和Maha）。为了探讨组织/植入物界面的炎症反应，还通过皮下植入BALB / C小鼠中的材料植入高达28天的抗蛋白酶。在这些分析中，在长期植入中没有在Meha或Maha中证明了显着的慢性炎症反应。

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  • 来源
    《RSC Advances》 |2020年第53期|共10页
  • 作者

    Zhang Lijun; DAmora Ugo; Ronca Alfredo; Li Yuanyuan; Mo Xiaoying; Zhou Fei; Yuan Mingzhou; Ambrosio Luigi; Wu Jun; Raucci Maria Grazia;

  • 作者单位

    Shenzhen Univ Affiliated Hosp 1 Shenzhen Peoples Hosp 2 Dept Burn &

    Plast Surg Shenzhen Peoples R China;

    CNR Inst Polymers Composites &

    Biomat Naples Italy;

    CNR Inst Polymers Composites &

    Biomat Naples Italy;

    Shenzhen Univ Affiliated Hosp 1 Shenzhen Peoples Hosp 2 Dept Burn &

    Plast Surg Shenzhen Peoples R China;

    Sun Yat Sen Univ Affiliated Hosp 1 Dept Burns Guangzhou Peoples R China;

    Sun Yat Sen Univ Affiliated Hosp 1 Dept Burns Guangzhou Peoples R China;

    Sun Yat Sen Univ Affiliated Hosp 1 Dept Burns Guangzhou Peoples R China;

    CNR Inst Polymers Composites &

    Biomat Naples Italy;

    Shenzhen Univ Affiliated Hosp 1 Shenzhen Peoples Hosp 2 Dept Burn &

    Plast Surg Shenzhen Peoples R China;

    CNR Inst Polymers Composites &

    Biomat Naples Italy;

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