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Mass spectrometric analysis of lipid A obtained from the lipopolysaccharide of Pasteurella multocida

机译：山梨酱脂肪糖脂质脂质A的质谱分析

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摘要

Haemorrhagic septicaemia is mainly caused by an opportunistic pathogen, Pasteurella multocida , a major threat to the livestock dependent economies. The main endotoxins are lipopolysaccharides. The lipid A, a key pathogenic part of lipopolysaccharides, anchors it into the bacterial cell membrane. Hence, profiling of the lipid A is important to understand toxicity of this pathogen. Despite a significant progress made on glycan analyses of core regions of lipopolysaccharides from various P. multocida strains, the structure of lipid A has not been reported yet. The lipid A of P. multocida type B:2 was analyzed using ESI-MS/MS to identify the acylation patterns, number and length of various acyl fatty acids, phosphorylation level and lipid A modifications. The MS ~( n ) data revealed the existence of multiple lipid A variants, i.e. mono and bisphosphorylated hepta-, hexa-, penta- and tetra-acylated structures, decorated with varied levels of 4-amino-4-deoxy- L -arabinose (Ara4N) on C-1 and/or C-4′ phosphate groups of proximal and distal glucosamine lipid A backbone. The detailed mass spectrometric analyses revealed that even within the same class, lipid A exhibits several sub-variant structures. A primary and secondary myristoylation at C-2, C-3, C-2′ and C-3′ was observed in all variants except hepta-acylated lipid A that carried a secondary palmitate at C-2 position. The lipid A profiling described herein, may contribute in exploring the mechanisms involved in endotoxicity of P. multocida type B:2 in haemorrhagic septicaemia disease.
机译：出血性败血症主要是由机会主义病原体，巴斯特氏菌多迪达引起的，这是对畜牧业依赖经济的重大威胁。主要内毒素是脂多糖。脂质A，脂多糖的关键致病部分，将其锚固到细菌细胞膜中。因此，脂质A的分析对于了解该病原体的毒性是重要的。尽管对来自各种P.多型菌株的脂多糖核心区的核心区进行了重大进展，但尚未报告脂质A的结构。使用ESI-MS / MS分析P. Multocida型B：2的脂质A，以鉴定各种酰基脂肪酸，磷酸化水平和脂质的酰化图案，数量和长度。 MS〜（n）数据显示，存在多种脂质的变体，即单磷和双磷酸化的庚酰基，六肟，五戊化和四酰化结构，用不同水平的4-氨基-4-脱氧-ARAbinose装饰（ARA4N）在C-1和/或C-4'磷酸盐基团上的近端和远端葡萄糖胺脂质的骨干。详细的质谱分析显示，即使在同一类内，脂质A也表现出几种副变体结构。在除了庚基 - 酰化脂质A之外的所有变体中观察到C-2，C-3，C-2'和C-3'的初级和次级多核，其在C-2位置携带二次棕榈酸酯。本文描述的脂质的脂质可能有助于探索患有血液血症疾病中P. Multocida型B：2的内毒性的机制。

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