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Mass spectrometric analysis of lipid A obtained from the lipopolysaccharide ofPasteurella multocida

机译：从Pstecolla multocada脂多糖中获得的脂质A的质谱分析

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摘要

Haemorrhagic septicaemia is mainly caused by an opportunistic pathogen,Pasteurella multocida, a major threat to the livestock dependent economies. The main endotoxins are lipopolysaccharides. The lipid A, a key pathogenic part of lipopolysaccharides, anchors it into the bacterial cell membrane. Hence, profiling of the lipid A is important to understand toxicity of this pathogen. Despite a significant progress made on glycan analyses of core regions of lipopolysaccharides from variousP. multocidastrains, the structure of lipid A has not been reported yet. The lipid A ofP. multocidatype B:2 was analyzed using ESI-MS/MS to identify the acylation patterns, number and length of various acyl fatty acids, phosphorylation level and lipid A modifications. The MS(n)data revealed the existence of multiple lipid A variants,i.e.mono and bisphosphorylated hepta-, hexa-, penta- and tetra-acylated structures, decorated with varied levels of 4-amino-4-deoxy-l-arabinose (Ara4N) on C-1 and/or C-4 ' phosphate groups of proximal and distal glucosamine lipid A backbone. The detailed mass spectrometric analyses revealed that even within the same class, lipid A exhibits several sub-variant structures. A primary and secondary myristoylation at C-2, C-3, C-2 ' and C-3 ' was observed in all variants except hepta-acylated lipid A that carried a secondary palmitate at C-2 position. The lipid A profiling described herein, may contribute in exploring the mechanisms involved in endotoxicity ofP. multocidatype B:2 in haemorrhagic septicaemia disease.
机译：出血性败血症主要是由机会主义病原体，巴斯特氏菌多迪达引起的，这是对畜牧业依赖经济的重大威胁。主要内毒素是脂多糖。脂质A，脂多糖的关键致病部分，将其锚固到细菌细胞膜中。因此，脂质A的分析对于了解该病原体的毒性是重要的。尽管对来自各种脂多糖的核心区核心区域进行了重大进展。多截面，尚未报告脂质A的结构。脂质A OFP。使用ESI-MS / MS分析多型型B：2，以鉴定各种酰基脂肪酸，磷酸化水平和脂质的酰化图案，数量和长度。 MS（n）数据显示，存在多种脂质的变体，Iemono和双磷酸化的庚酸，六曲，五戊基和四酰基化结构，用不同水平的4-氨基-4-脱氧-L-阿拉伯糖（ ARA4N）在近端和远端葡糖胺脂质的C-1和/或C-4'磷酸盐组上骨干。详细的质谱分析显示，即使在同一类内，脂质A也表现出几种副变体结构。在除了庚基 - 酰化脂质A之外的所有变体中观察到C-2，C-3，C-2'和C-3'的初级和次级多核，其在C-2位置携带二次棕榈酸酯。本文描述的脂质的脂质可能有助于探索中毒性的涉及的机制。多型型B：2在出血性败血症疾病中。

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  • 来源
    《RSC Advances》 |2020年第51期|共17页
  • 作者

    Tawab Abdul; Akbar Noor; Hasssan Mujtaba; Habib Fazale; Ali Aamir; Rahman Moazur; Jabbar Abdul; Rauf Waqar; Iqbal Mazhar;

  • 作者单位

    Natl Inst Biotechnol &

    Genet Engn NIBGE Hlth Biotechnol Div POB 577 Jhang Rd Faisalabad 38000 Pakistan;

    Natl Inst Biotechnol &

    Genet Engn NIBGE Hlth Biotechnol Div POB 577 Jhang Rd Faisalabad 38000 Pakistan;

    Natl Inst Biotechnol &

    Genet Engn NIBGE Hlth Biotechnol Div POB 577 Jhang Rd Faisalabad 38000 Pakistan;

    Natl Inst Biotechnol &

    Genet Engn NIBGE Hlth Biotechnol Div POB 577 Jhang Rd Faisalabad 38000 Pakistan;

    Natl Inst Biotechnol &

    Genet Engn NIBGE Hlth Biotechnol Div POB 577 Jhang Rd Faisalabad 38000 Pakistan;

    Natl Inst Biotechnol &

    Genet Engn NIBGE Hlth Biotechnol Div POB 577 Jhang Rd Faisalabad 38000 Pakistan;

    Natl Inst Biotechnol &

    Genet Engn NIBGE Hlth Biotechnol Div POB 577 Jhang Rd Faisalabad 38000 Pakistan;

    Natl Inst Biotechnol &

    Genet Engn NIBGE Hlth Biotechnol Div POB 577 Jhang Rd Faisalabad 38000 Pakistan;

    Natl Inst Biotechnol &

    Genet Engn NIBGE Hlth Biotechnol Div POB 577 Jhang Rd Faisalabad 38000 Pakistan;

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