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Easily fabricated monolithic fluoropolymer chips for sensitive long-term absorbance measurement in droplet microfluidics

机译:易于制造整体含氟聚合物芯片,用于液滴微流体中的敏感长期吸光度测量

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Maintaining a hydrophobic channel surface is critical to ensuring long-term stable flow in droplet microfluidics. Monolithic fluoropolymer chips ensure robust and reliable droplet flow as their native fluorous surfaces naturally preferentially wet fluorocarbon oils and do not deteriorate over time. Their fabrication, however, typically requires expensive heated hydraulic presses that make them inaccessible to many laboratories. Here we describe a method for micropatterning and bonding monolithic fluoropolymer flow cells from a commercially available melt-processable fluoropolymer, Dyneon THV 500GZ, that only requires a standard laboratory oven. Using this technique, we demonstrate the formation of complex microstructures, specifically the fabrication of sensitive absorbance flow cells for probing droplets in flow, featuring path lengths up to 10 mm. The native fluorous channel surface means the flow cells can be operated over extended periods, demonstrated by running droplets continuously through a chip for 16 weeks.
机译:保持疏水性通道表面对于确保液滴微流体中的长期稳定流动是至关重要的。单片含氟聚合物芯片可确保鲁棒且可靠的液滴流,因为它们的天然氟表面自然优先湿氟碳油,并且随着时间的推移不会恶化。然而,它们的制造通常需要昂贵的加热液压机,使它们无法进入许多实验室。在这里,我们描述了一种用于从市售的熔融加工含氟聚合物,DYNON THV 500Gz的微型蚀刻和粘合整体含氟聚合物流动细胞的方法,该方法仅需要标准实验室烘箱。使用该技术,我们证明了复杂微结构的形成,特别是用于探测流动液滴的敏感吸光度流动细胞的制造,具有高达10mm的路径长度。本机氟沟道表面意味着流动电池可以在延长的时段中操作,通过连续地通过芯片连续地通过芯片进行16周来说明。

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