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首页> 外文期刊>RSC Advances >Electrochemical sensory detection of Sus scrofa mtDNA for food adulteration using hybrid ferrocenylnaphthalene diimide intercalator as a hybridization indicator
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Electrochemical sensory detection of Sus scrofa mtDNA for food adulteration using hybrid ferrocenylnaphthalene diimide intercalator as a hybridization indicator

机译：用杂交铁烯基萘二酰亚胺嵌入剂作为杂交指示剂的杂交铁烯基萘二酰亚胺掺杂的电化学感官检测

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In this study, an electrochemical DNA biosensor was developed based on the fabrication of silicon nanowires/platinum nanoparticles (SiNWs/PtNPs) on a screen-printed carbon electrode (SPCE) for the detection of Sus scrofa mitochondrial DNA (mtDNA) in food utilizing a new hybrid indicator, ferrocenylnaphthalene diimide (FND). The morphology and elemental composition of the SiNWs/PtNPs-modified SPCE was analyzed by field emission scanning electron microscopy (FESEM) combined with energy dispersive X-ray spectroscopy (EDX). Cyclic voltammetry (CV) was used to study the electrical contact between the PtNPs and the screen-printed working electrode through SiNWs, while electrochemical impedance spectroscopy (EIS) was used to measure the charge transfer resistance of the modified electrode. The results clearly showed that the SiNWs/PtNPs were successfully coated onto the electrode and the effective surface area for the SiNWs/PtNPs-modified SPCE was increased 16.8 times as compared with that of the bare SPCE. Differential pulse voltammetry used for the detection of porcine DNA with FND as an intercalator confirmed its specific binding to the double-stranded DNA (dsDNA) sequences. The developed biosensor showed a selective response towards complementary target DNA and was able to distinguish non-complementary and mismatched DNA oligonucleotides. The SiNWs/PtNPs-modified SPCE that was fortified with DNA hybridization demonstrated good linearity in the range of 3 × 10 ~(?9) M to 3 × 10 ~(?5) M ( R ~(2) = 0.96) with a detection limit of 2.4 × 10 ~(?9) M. A cross-reactivity study against various types of meat and processed food showed good reliability for porcine samples.
机译：在该研究中，基于在丝网印刷的碳电极（SPCE）上的硅纳米线/铂纳米颗粒（SINWS / PTNP）的制造来开发电化学DNA生物传感器，用于检测利用a的食物中的SU Scrofa线粒体DNA（MTDNA）。新型杂交指示器，铁烯基萘二酰亚胺（FND）。通过现场发射扫描电子显微镜（FeSEM）与能量分散X射线光谱（EDX）结合的SINWS / PTNPS改性SPCE的形态和元素组成。使用循环伏安法（CV）来研究PTNP和丝网印刷的工作电极通过SINWS之间的电接触，而电化学阻抗光谱（EIS）用于测量改性电极的电荷传递电阻。结果清楚地表明，与裸露的SPCE相比，SINWS / PTNP成功涂覆在电极上，并且SINWS / PTNPS改性SPCE的有效表面积增加了16.8倍。用于检测具有FND作为嵌入剂的猪DNA的差分脉冲伏安法证实其与双链DNA（DSDNA）序列的特异性结合。开发的生物传感器显示出对互补靶DNA的选择性响应，并且能够区分非互补和错配的DNA寡核苷酸。用DNA杂交强化的SINWS / PTNPS修饰的SPCE在3×10〜（α9）m至3×10〜（Δ5）m（r〜（2）= 0.96）的范围内显示出良好的线性。（R〜（2）= 0.96）检测限2.4×10〜（？9）M.对各种类型的肉类和加工食品的交叉反应性研究表现出猪样品的良好可靠性。

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