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首页> 外文期刊>RSC Advances >Purification, characterization, and hypoglycemic properties of eurocristatine from Eurotium cristatum spores in Fuzhuan brick tea
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Purification, characterization, and hypoglycemic properties of eurocristatine from Eurotium cristatum spores in Fuzhuan brick tea

机译：富株师砖茶中欧洲克里斯坦孢子的纯化，表征和低血糖特性

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Fuzhuan brick tea (FBT) is a Chinese dark tea that is famous for its significant health benefits, in which Eurotium cristatum ( E. cristatum ) strains play a vital role in its postfermentation process. In this study, eurocristatine with hypoglycemic activity was discovered for the first time and purified from the spores of E. cristatum growing in FBT. Eurocristatine (98%) was obtained by D-101 macroporous resin-based column chromatography and preparative high performance liquid chromatography (HPLC) with a C _(18) column as the stationary phase and 35% acetonitrile in ultrapure water as the mobile phase. Hypoglycemic activity in a Hep-G2 cell hypoglycemic model was used as a screening indicator during purification. The chemical structure of eurocristatine was characterized by ESI/MS, ~(1) H NMR and ~(13) C NMR analyses. The antidiabetic effects of eurocristatine were verified in high-fat diet/streptozocin-induced type 2 diabetes mellitus (T2DM) rats. The results showed that eurocristatine significantly reduced fasting blood glucose. Our study demonstrated that eurocristatine, as a newly discovered hypoglycemic active substance, could be considered a potential candidate for the treatment of diabetes and its complications.
机译：Fuzhuan砖茶（FBT）是中国黑茶，以其重大的健康益处而闻名，其中Eurotium Cristatum（E. Cristatum）菌株在其介绍过程中发挥着重要作用。在这项研究中，首次发现了具有降血糖活性的Eurocristatine，并从FBT生长的E. Cristatum的孢子净化。通过D-101大孔树脂基色谱法和制备高效液相色谱（HPLC）获得Eurocristatine（98％），用C _（18）塔作为固定相和35％乙腈作为流动相。 HEP-G2细胞降血糖模型中的降血糖活性用作纯化期间的筛选指示剂。 Eutocristine的化学结构的特征在于ESI / MS，〜（1）H NMR和〜（13）C NMR分析。 Eurocristatine的抗糖尿病效果在高脂饮食/链脲素诱导的2型糖尿病（T2DM）大鼠中验证。结果表明，埃菲尔汀显着减少了血糖的空腹血糖。我们的研究表明，Eurocristine作为一种新发现的降血糖活性物质，可以被认为是治疗糖尿病的潜在候选者及其并发症。

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