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首页> 外文期刊>RSC Advances >Cytotoxic effect of green silver nanoparticles against ampicillin-resistant Klebsiella pneumoniae
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Cytotoxic effect of green silver nanoparticles against ampicillin-resistant Klebsiella pneumoniae

机译：绿银纳米粒子对氨苄青霉素抗性Klebsiella肺炎的细胞毒性作用

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Considering the harmful effects and high spread of drug-resistant Klebsiella pneumoniae , many researchers have been trying to produce new antibacterial agents to combat the emergence of multidrug-resistant (MDR) strains of this bacterium. Recent progress in the nanomedicine field has provided opportunities for synthesizing unique nanoagents to battle MDR bacteria by targeting virulence and resistance signalling. The biocidal effects of 14.9 nm silver nanoparticles fabricated using Nostoc sp. Bahar M (N-SNPs) and AgNO _(3) were examined against drug-resistant K. pneumoniae using the agar well diffusion method. Transmission electron microscopy (TEM) was used to detect the ultrastructural changes caused by N-SNPs and AgNO _(3) . To address the mode of action of N-SNPs and AgNO _(3) , CAT, GPx, LDH and ATPase levels were assessed. The toxicity of N-SNPs and AgNO _(3) was evaluated against the mfD , flu , hly , 23 S , hns , hcp -1, VgrG -1 and VgrG -3 genes as well as cellular proteins. N-SNPs showed the greatest inhibitory activity against K. pneumoniae , with MIC and MBC values of 0.9 and 1.2 mg mL ~(?1) , respectively. Furthermore, N-SNPs and AgNO _(3) induced apoptotic features, including cell shrinkage and cell atrophy. N-SNPs were more potent bactericidal compounds than AgNO _(3) , causing increased leakage of LDH and GPx activities and depletion of ATPase and CAT activities, resulting in induced oxidative stress and metabolic toxicity. Compared to AgNO _(3) , N-SNPs exhibited the highest toxicity towards the selected genes and the greatest damage to bacterial proteins. N-SNPs were the most potent agents that induced bacterial membrane damage, oxidative stress and disruption of biomolecules such as DNA and proteins. N-SNPs may be used as effective nanodrugs against MDR bacteria.
机译：考虑到耐药性Klebsiella肺炎的有害效果和高差，许多研究人员一直试图产生新的抗菌剂，以应对这种细菌的多药（MDR）菌株的出现。纳米美床领域的最近进展为合成独特的纳米代理人来通过靶向毒力和阻力信令来合成独特的纳米蛋白。使用Nostoc SP制造14.9 nm银纳米粒子的杀生物效应。使用琼脂孔扩散法检查抗毒性K.肺炎的毒性K.抗毒性K.Aharm（N-SNP）和AgNo _（3）。透射电子显微镜（TEM）用于检测由N-SNP和AGNO _（3）引起的超微结构变化。为了解决N-SNP和AgNO _（3）的作用方式，评估CAT，GPX，LDH和ATP酶水平。对MFD，流感，HLY，23 S，HNS，HCP -1，VGRG -1和VGRG -3基因以及细胞蛋白评估N-SNP和AgNO _（3）的毒性。 N-SNP分别显示出对K.肺炎的最大抑制活性，MIC和MBC值分别为0.9和1.2mg mL〜（α1）。此外，N-SNP和AgNo _（3）诱导凋亡特征，包括细胞收缩和细胞萎缩。 N-SNP比Agno _（3）更有效的杀菌化合物，导致LDH和GPX活性泄漏和ATP酶和猫活动的耗尽，导致诱导氧化应激和代谢毒性。与AgNo _（3）相比，N-SNP表现出对所选基因的最高毒性，以及对细菌蛋白最大的损害。 N-SNP是最有效的药剂，诱导细菌膜损伤，氧化应激和生物分子的破坏，如DNA和蛋白质。 N-SNP可以用作针对MDR细菌的有效纳米颗粒。

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