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Cytotoxic effect of green silver nanoparticles against ampicillin-resistantKlebsiella pneumoniae

机译：绿银纳米粒子对氨苄青霉素抗性杆菌菌肺炎的细胞毒性作用

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Considering the harmful effects and high spread of drug-resistantKlebsiella pneumoniae, many researchers have been trying to produce new antibacterial agents to combat the emergence of multidrug-resistant (MDR) strains of this bacterium. Recent progress in the nanomedicine field has provided opportunities for synthesizing unique nanoagents to battle MDR bacteria by targeting virulence and resistance signalling. The biocidal effects of 14.9 nm silver nanoparticles fabricated usingNostocsp. Bahar M (N-SNPs) and AgNO(3)were examined against drug-resistantK. pneumoniaeusing the agar well diffusion method. Transmission electron microscopy (TEM) was used to detect the ultrastructural changes caused by N-SNPs and AgNO3. To address the mode of action of N-SNPs and AgNO3, CAT, GPx, LDH and ATPase levels were assessed. The toxicity of N-SNPs and AgNO(3)was evaluated against themfD,flu,hly, 23S,hns,hcp-1,VgrG-1 andVgrG-3 genes as well as cellular proteins. N-SNPs showed the greatest inhibitory activity againstK. pneumoniae, with MIC and MBC values of 0.9 and 1.2 mg mL(-1), respectively. Furthermore, N-SNPs and AgNO(3)induced apoptotic features, including cell shrinkage and cell atrophy. N-SNPs were more potent bactericidal compounds than AgNO3, causing increased leakage of LDH and GPx activities and depletion of ATPase and CAT activities, resulting in induced oxidative stress and metabolic toxicity. Compared to AgNO3, N-SNPs exhibited the highest toxicity towards the selected genes and the greatest damage to bacterial proteins. N-SNPs were the most potent agents that induced bacterial membrane damage, oxidative stress and disruption of biomolecules such as DNA and proteins. N-SNPs may be used as effective nanodrugs against MDR bacteria.
机译：考虑到药物 - 抵抗抗杆素肺炎的有害影响和高蔓延，许多研究人员一直试图产生新的抗菌剂，以应对这种细菌的多药（MDR）菌株的出现。纳米美床领域的最近进展为合成独特的纳米代理人来通过靶向毒力和阻力信令来合成独特的纳米蛋白。使用诺斯科普斯的14.9 nm银纳米粒子的杀生物效应。检查Bahar M（N-SNP）和Agno（3）针对药物抗药性。肺炎琼脂扩散方法。透射电子显微镜（TEM）用于检测由N-SNP和AGNO3引起的超微结构变化。为了解决N-SNP和AgNO3的作用方式，评估猫，GPX，LDH和ATP酶水平。对N-SNP和AgNO（3）的毒性进行评估对OFFD，流感，HLY，23s，HNS，HCP-1，VGRG-1和vgrg-3基因以及细胞蛋白。 N-SNP展示了最大的抑制活动逆后。肺炎，MIC和MBC值分别为0.9和1.2mg mL（-1）。此外，N-SNP和AgNO（3）诱导凋亡特征，包括细胞收缩和细胞萎缩。 N-SNP比AgNO 3更有效的杀菌化合物，导致LDH和GPX活性泄漏和ATP酶和猫活动的耗尽，导致诱导氧化应激和代谢毒性。与AgNO 3相比，N-SNP对所选基因的最高毒性和对细菌蛋白质最大的损害。 N-SNP是最有效的药剂，诱导细菌膜损伤，氧化应激和生物分子的破坏，如DNA和蛋白质。 N-SNP可以用作针对MDR细菌的有效纳米颗粒。

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  • 来源
    《RSC Advances》 |2020年第36期|共11页
  • 作者

    Hamida Reham Samir; Ali Mohamed Abdelaal; Goda Doaa A.; Khalil Mahmoud Ibrahim; Redhwan Alya;

  • 作者单位

    Alexandria Univ Fac Sci Dept Zool Mol Biol Unit Alexandria Egypt;

    King Saud Univ Coll Food &

    Agr Sci Dept Plant Prod Biotechnol Unit Riyadh Saudi Arabia;

    City Sci Res &

    Technol Applicat SRTA City Bioproc Dev Dept Genet Engn &

    Biotechnol Res Inst GEBRI Alexandria Egypt;

    Alexandria Univ Fac Sci Dept Zool Mol Biol Unit Alexandria Egypt;

    Princess Nourah Bint Abdulrahman Univ Coll Hlth &

    Rehabil Sci Dept Hlth Riyadh Saudi Arabia;

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