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首页> 外文期刊>RSC Advances >The use of principle component analysis and MALDI-TOF MS for the differentiation of mineral forming Virgibacillus and Bacillus species isolated from sabkhas
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The use of principle component analysis and MALDI-TOF MS for the differentiation of mineral forming Virgibacillus and Bacillus species isolated from sabkhas

机译：使用原理成分分析和MALDI-TOF MS进行矿物质成型VIRGIBACILLUS的分化和从SABKHAS中分离的芽孢杆菌物种

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Occurrence of mineral forming and other bacteria in mats is well demonstrated. However, their high diversity shown by ribotyping has not been explained, although it could explain the diversity of formed minerals. Common biomarkers as well as phylogenic relationships are useful tools for clustering the isolates and the prediction of their potential role in the natural niche. In this study, a combination of MALDI-TOF MS with PCA was shown to be a powerful tool to categorize 35 mineral forming bacterial isolates isolated from Dohat Faishakh sabkha, northwest of Qatar (23 from decaying mats and 12 from living ones). The 23 strains from decaying mats belong to the Virgibacillus genus as identified by ribotyping and are shown to be highly involved in the formation of protodolomite and a diversity of minerals. They were used as internal references for the categorization of sabkha bacteria. Combination of the isolation of bacteria on selective mineral forming media, their MALDI TOF MS protein profiling and PCA analysis established their relationship in a phylloproteomic dendrogram based on protein biomarkers including m / z 4905, 3265, 5240, 6430, 7765, and 9815. PCA analysis clustered the studied isolates into 3 major clusters, showing strong correspondence to the 3 phylloproteomic groups that were established by the dendrogram. Both clustering analysis means have evidently demonstrated a relationship between known Virgibacillus strains and other related bacteria based on profiling of their synthesized proteins. Thus, larger populations of bacteria in mats can be easily screened for their potential to exhibit certain activities, which is of ecological, environmental and biotechnological significance.
机译：垫子中矿物成型和其他细菌的发生得很好。然而，尤科划分所显示的高多样性尚未解释，尽管它可以解释成型矿物质的多样性。常见的生物标志物以及系统发育关系是用于聚类分离物的有用工具和预测天然利基中的潜在作用。在这项研究中，MALDI-TOF MS与PCA的组合被证明是一种强大的工具，将35个矿物成型细菌分离株分类为从卡塔尔西北部的Dohat Faishakh Sabkha分离（23来自腐朽的垫子，从生活中有12个）。腐烂垫的23个菌株属于通过核糖型鉴定的Virgibacillus Genus，并且被证明是强制性的形成和多样性的矿物质。它们被用作对Sabkha细菌分类的内部参考。细菌分离对选择性矿物形成培养基的组合，它们的MALDI TOF MS蛋白质分析和PCA分析基于包括M / Z 4905,3265,5240,6430,7765和9815的蛋白质​​生物标志物在文学生物标志物中建立了它们的关系.PCA分析将研究的分离物聚集成3个主要簇，显示出对由树木图建立的3个字节蛋白组基团的强对应关系。聚类分析装置都显着证明了根据合成蛋白质的分析的已知维虫酸菌株和其他相关细菌之间的关系。因此，可以容易地筛选垫子中的细菌的较大群体，以潜在表现出某些活动，这是生态，环境和生物技术的意义。

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