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Screening and characterisation of CdTe/CdS quantum dot-binding peptides for material surface functionalisation

机译：用于材料表面官能化的CDTE / CDS量子点结合肽的筛选与表征

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Quantum dots (QDs) are promising nanomaterials due to their unique photophysical properties. For them to be useful in biological applications, the particle surface generally needs to be conjugated to biological molecules, such as antibodies. In this study, we screened CdTe/CdS QD-binding peptides from a phage display library as linkers for simple and bio-friendly QD modification. Among five QD-binding peptide candidates, a series of truncated peptides designed from two high-affinity peptides were subjected to an array-based binding assay with QDs to assess their functional core sequences and characteristics. Linking these isolated, shortened peptides (PWSLNR and SGVYK) with an antibody-binding peptide (NKFRGKYK) created dual-functional peptides that are capable of QD surface functionalisation by antibodies. Consequently, the dual-functional peptides could mediate anti-CD9 antibody functionalisation onto CdTe/CdS QD surface; CD9 protein imaging of cancer cells was also demonstrated. Our proposed peptides offer an effective vehicle for QD surface functionalisation in biological applications.
机译：由于它们独特的光学性质，量子点（QDS）是有前途的纳米材料。为了它们在生物学应用中可用，颗粒表面通常需要与生物分子（例如抗体）缀合。在该研究中，我们将CdTe / Cds QD结合肽从噬菌体展示文库中筛选为用于简单和生物友好的QD改性的接头。在五个QD结合肽候选中，用QDS对由两个高亲和力肽设计的一系列截短的肽从两个高亲和力肽设计为基于阵列的结合测定，以评估其功能核序列和特性。将这些隔离的肽（PWSLNR和SGVYK）与抗体结合肽（NKFRGKYK）连接，产生了能够通过抗体的QD表面官能化的双官能肽。因此，双官能肽可以将抗CD9抗体官能介导到CDTE / CDS QD表面上;还证明了CD9蛋白质成像癌细胞。我们所提出的肽为生物应用中的QD表面官能化提供有效的载体。

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