An improved 4′-aminomethyltrioxsalen-based nucleic acid crosslinker for biotinylation of double-stranded DNA or RNA

Kevin Wielenberg; Miao Wang; Min Yang; Abdullah Ozer; John T. Lis; Hening Lin

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An improved 4′-aminomethyltrioxsalen-based nucleic acid crosslinker for biotinylation of double-stranded DNA or RNA

机译：一种改进的4'-氨基甲基三氧基Xalen基核酸交联剂，用于双链DNA或RNA的生物素化

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Nucleic acid crosslinkers that covalently join complementary strands of DNA/RNA have applications in both pharmaceuticals and as biochemical probes. Psoralen is a popular crosslinking moiety that reacts with double stranded DNA and RNA upon exposure to longwave UV light. The commercially available compound EZ-link psoralen-PEG3-biotin has been used in numerous studies to crosslink DNA and double-stranded RNA for genome-wide investigations. Here we present a new probe, AP3B , which uses the psoralen derivative, 4′-aminomethyltrioxsalen, to crosslink and biotinylate nucleic acids. We show that AP3B is 4 to 5 times more effective at labeling DNA in cells and produces a comparable number of crosslinks with over 100 times less compound and less exposure to UV light in vitro than EZ-link psoralen-PEG3-biotin.

机译：共价加入DNA / RNA互补链的核酸交联剂在药物和生物化学探针中具有应用。 Psoralen是一种普遍的交联部分，其在暴露于长波UV光时与双链DNA和RNA反应。市售的化合物EZ-Link Psoralen-PEG3-Biotin已用于许多研究中，用于交联DNA和用于基因组的研究的双链RNA。在这里，我们提出了一种新的探针，AP3B，其使用牛奶醛衍生物，4'-氨基甲基三氧基Xalen与交联和生物素化核酸。我们表明AP3B在细胞中标记DNA时，AP3B是4至5倍，并产生比EZ-Link PSORALEN-PEG3-BIOTIN在体外超过100倍的交联的交联的交联。

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《RSC Advances》 |2020年第65期|共5页
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Kevin Wielenberg; Miao Wang; Min Yang; Abdullah Ozer; John T. Lis; Hening Lin;
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机译：使用基于肽核酸和双链DNA杂交的电化学DNA生物传感器直接检测和区分对应于丙型肝炎病毒基因型3a的双链寡核苷酸
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机译：一种改进的4'-氨基甲基三氧基Xalen基核酸交联剂，用于双链DNA或RNA的生物素化
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An improved 4′-aminomethyltrioxsalen-based nucleic acid crosslinker for biotinylation of double-stranded DNA or RNA

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