More than one way to bind to cholesterol: atypical variants of membrane-binding domain of perfringolysin O selected by ribosome display

Aleksandra ?akanovi?; Nace Kranjc; Ne?a Omersa; Marjetka Podobnik; Gregor Anderluh

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More than one way to bind to cholesterol: atypical variants of membrane-binding domain of perfringolysin O selected by ribosome display

机译：与胆固醇结合的多种方式：由核糖体显示器选择的蜂窝蛋白o的膜结合结构域的非典型变体

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Herein, we report a high-throughput approach for the selection of peripheral protein domains that bind specifically to cholesterol in lipid membranes. We discovered variants of perfringolysin O, with non-conserved amino acid substitutions at regions crucial for cholesterol recognition, demonstrating an unprecedented amino acid sequence variability with binding ability for cholesterol. The developed approach provides an effective platform for a comprehensive study of protein lipid interactions.

机译：在此，我们报告了用于选择与脂质膜中胆固醇特异性结合的外周蛋白质结构域的高通量方法。我们发现了灌注酚素O的变体，在胆固醇识别的区域下具有非保守氨基酸取代，证明了前所未有的氨基酸序列可变性，胆固醇的结合能力。开发方法提供了综合研究蛋白质脂质相互作用的有效平台。

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《RSC Advances》 |2020年第63期|共5页
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Aleksandra ?akanovi?; Nace Kranjc; Ne?a Omersa; Marjetka Podobnik; Gregor Anderluh;
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1. Modifications in Perfringolysin O Domain 4 Alter the Cholesterol Concentration Threshold Required for Binding [J] . Benjamin B. Johnson, Paul C. Moe, David Wang, Biochemistry . 2012,第16期

机译：Perfringolysin O域4的修饰会改变结合所需的胆固醇浓度阈值
2. Positive allosteric modulators of lecithin: Cholesterol acyltransferase adjust the orientation of the membrane-binding domain and alter its spatial free energy profile [J] . Akseli Niemela¨, Artturi Koivuniemi PLoS Computational Biology . 2021,第3期

机译：卵磷脂的正变构调节剂：胆固醇酰基转移酶调节膜结合结构域的取向，改变其空间自由能曲线
3. HIV-1 variants with a single-point mutation in the gp41 pocket region exhibiting different susceptibility to HIV fusion inhibitors with pocket- or membrane-binding domain [J] . LuL., TongP., YuX., Biochimica et biophysica acta. Biomembranes . 2012,第12期

机译：在gp41口袋区域具有单点突变的HIV-1变体对带有口袋或膜结合域的HIV融合抑制剂表现出不同的敏感性
4. Simple and Effective Selection of Cyclic Peptides by Ribosome Display with PUREfrex [C] . Kanehisa Kojoh, Kumiko Tsuihiji PEGS . 2015

机译：用镶骨显示用乙型纤维素的循环肽的简单有效选择
5. NMR investigations of solution and membrane-binding properties of synaptotagmin1 C2A domain. [D] . Bhowmik, Anshu Narayan. 2008

机译：核磁共振研究溶液和膜结合性质的突触小蛋白1 C2A域。
6. Selective binding of perfringolysin O derivative to cholesterol-rich membrane microdomains (rafts) [O] . A. A. Waheed, Yukiko Shimada, Harry F. G. Heijnen, 2001

机译：穿孔球菌溶血素O衍生物与多肽的选择性结合富含胆固醇的膜微区（筏）
7. More than one way to bind to cholesterol: atypical variants of membrane-binding domain of perfringolysin O selected by ribosome display [O] . Aleksandra Šakanović, Nace Kranjc, Neža Omersa, 2020

机译：与胆固醇结合的多种方式：由核糖体显示器选择的蜂窝蛋白o的膜结合结构域的非典型变体

More than one way to bind to cholesterol: atypical variants of membrane-binding domain of perfringolysin O selected by ribosome display

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