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首页> 外文期刊>RSC Advances >The therapeutic effect of Bletilla striata extracts on LPS-induced acute lung injury by regulation of inflammation and oxidation
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The therapeutic effect of Bletilla striata extracts on LPS-induced acute lung injury by regulation of inflammation and oxidation

机译：通过调节炎症和氧化对LPS诱导急性肺损伤的Bletilla Striata提取物的治疗作用

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Bletilla striata , a widely used traditional herbal medicine, has been shown to exhibit various biological activities but its antioxidative and anti-inflammatory activities have not been well studied. In this study, we isolated the active ingredients from B. striata , and identified the ingredient structures from the highest antioxidative fraction BM60 using HPLC-ESI-HRMS and then investigated the antioxidative and anti-inflammatory responses and the underlying mechanisms of fraction BM60 both in vitro and in vivo . The BM60 treatment reduced the production of NO in NR8383 macrophages. In addition, acute lung inflammation was induced in mice by intratracheal instillation of lipopolysaccharide (3.0 mg kg ~(?1) ), and treatments with BM60 at the doses of 35, 70, 140 mg kg ~(?1) significantly reduced macrophages and neutrophils in the bronchoalveolar lavage fluid (BALF) and markedly ameliorated lung wet-to-dry weight (W/D) ratios, myeloperoxidase (MPO) activity and pulmonary histopathological conditions. Moreover, the treatment might be attributed to the down-regulations of neutrophil infiltration, malondialdehyde (MDA) and up-regulations of superoxide dismutase (SOD) in lung tissues. In addition, the BM60 treatment inhibited the infiltration of inflammatory cells and tumour necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) production. The results from histopathological and immunochemical analyses confirmed the above biochemical assay data. The preliminary mechanistic study indicated that BM60 suppressed LPS-induced nuclear factor-κB (NF-κB) activation in a dose-dependent manner. Therefore, this work prompted further evaluation of B. striata to discover individual active ingredients for preventing acute lung injury in the future.
机译：已经显示出广泛使用的传统草药的Bletilla Striata，已经显示出各种生物活性，但其抗氧化和抗炎活动尚未得到很好的研究。在该研究中，我们将活性成分与B.Striata分离，并使用HPLC-ESI-HRMS从最高抗氧化级分BM60中鉴定了成分结构，然后研究了抗氧化和抗炎反应以及馏分BM60的潜在机制体外和体内。 BM60治疗减少了NR8383巨噬细胞的生产。此外，通过脂多糖的脑内滴注（3.0mg kg〜（α1））诱导急性肺炎症，并在35,70,140mg kg〜（α1）的剂量下用BM60的处理显着降低巨噬细胞和中性粒细胞在支气管肺泡灌洗液（BALF）中，并显着改善肺部湿对干重（W / D）比率，髓氧化酶（MPO）活性和肺组织病理病症。此外，该治疗可能归因于肺组织中的中性粒细胞浸润，丙二醛（MDA）和超氧化物歧化酶（SOD）的上氧化酶抑制（SOD）的下降规律。此外，BM60治疗抑制炎症细胞和肿瘤坏死因子-α（TNF-α），白细胞介素-1β（IL-1β）和白细胞介素-6（IL-6）的产生的渗透。组织病理学和免疫化学分析的结果证实了上述生物化学测定数据。初步机械研究表明，BM60以剂量依赖性方式抑制了LPS诱导的核因子-κB（NF-κB）活化。因此，这项工作促进了对B. Striata的进一步评估，以发现未来预防急性肺损的个体活性成分。

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