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首页> 外文期刊>RSC Advances >Probing and visualizing the heterogeneity of fiber cell wall deconstruction in sugar maple (Acer saccharum) during liquid hot water pretreatment
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Probing and visualizing the heterogeneity of fiber cell wall deconstruction in sugar maple (Acer saccharum) during liquid hot water pretreatment

机译：液体热水预处理期间糖枫（Acer Saccharum）纤维细胞壁解构的异质性探讨和可视化

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Liquid hot water (LHW) pretreatment is an effective method to improve enzymatic digestibility of lignocellulosic feedstock by removing hemicellulose/lignin. To better understand how LHW pretreatment reduces plant cell wall recalcitrance, we applied a combined approach using multiple microscopic techniques and chemometric methods to monitor microstructural and topochemical changes in the fiber cell wall of sugar maple ( Acer saccharum ). The heterogeneity of deconstruction in various cell wall layers was easily visualized based on chemical characterizations through confocal Raman microscopy, combining with principal component analysis and cluster analysis. Interestingly, after LHW pretreatment, the S2 layer was differentiated into two regions, namely a heavy-damaged region (outer and thin inner S2) with more polysaccharides removed, and a light-damaged region (middle S2) which still remained relatively intact. Our results have established a direct correlation between microstructural and topochemical changes of a cell wall following LHW pretreatment. The removal of polysaccharides (mainly hemicelluloses) rather than lignin played a critical role in the visible damage of the cell wall, like cavities, gaps and collapses.
机译：液体热水（LHW）预处理是通过除去半纤维素/木质素来改善木质纤维素原料的酶促消化率的有效方法。为了更好地了解LHW预处理如何减少植物细胞壁克批分法，我们使用多种微观技术和化学计量方法应用了组合方法，以监测糖枫（Acer Saccharum）纤维细胞壁中的微观结构和TopeChemical变化。基于通过共焦拉曼显微镜的化学表征，可以容易地可视化各种细胞壁层的非均匀性，与主成分分析和聚类分析相结合。有趣的是，在LHW预处理之后，将S2层分为两个区域，即除去更多多糖的重损伤区域（外部和薄的内部S2），并且仍然保持相对完整的透明区域（中间S2）。我们的结果建立了LHW预处理后细胞壁的微观结构和类别化学变化之间的直接相关性。去除多糖（主要是半纤维素）而不是木质素在细胞壁的可见损伤中发挥着关键作用，如空腔，间隙和坍塌。

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