Novel β-galactosynthase–β-mannosynthase dual activity of β-galactosidase from Aspergillus oryzae uncovered using monomer sugar substrates

R. S. Tan; H. Hinou; S.-I. Nishimura

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Novel β-galactosynthase–β-mannosynthase dual activity of β-galactosidase from Aspergillus oryzae uncovered using monomer sugar substrates

机译：用单体糖基材未覆盖来自曲霉属的β-半乳糖苷酶的新型β-半乳糖糖苷酶-β-甘露糖苷酶的双活性

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A novel β-galactosynthase–β-mannosynthase dual-activity of β-galactosidase from Aspergillus oryzae was uncovered, for the first time, using free monosaccharide substrates. The enzyme successfully converted galactose and mannose monomer sugars efficiently into Gal-β(1–6)-Gal and Man-β(1–6)-Man, respectively. The discovery could potentially revolutionize chemoenzymatic glycan and non-digestible oligosaccharide (NDO) syntheses.

机译：首次使用游离单糖基质，首次揭示来自曲霉属植物的β-半乳糖苷酶-β-甘露糖苷酶的β-半乳糖苷酶的双活性。该酶分别成功地将半乳糖和甘露糖单体糖转化为Gal-β（1-6）-gal和Man-β（1-6） - 曼。发现可能透过化学酶聚糖和不可消化的低聚糖（NDO）合成。

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《RSC Advances》 |2016年第56期|共4页
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R. S. Tan; H. Hinou; S.-I. Nishimura;
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1. Novel beta-galactosynthase-beta-mannosynthase dual activity of beta-galactosidase from Aspergillus oryzae uncovered using monomer sugar substrates [J] . Tan R. S., Hinou H., Nishimura S. -I. RSC Advances . 2016,第56期

机译：来自曲霉属植物的β-半乳糖苷酶的新型β-半乳糖糖苷酶 - β-甘露糖苷酶的双重活性使用单体糖基材未覆盖
2. Enzymatic removal of flatulence-inducing sugars in chickpea milk using free and polyvinyl alcohol immobilized alpha-galactosidase from Aspergillus oryzae [J] . Patil AG, Kote NV, Mulimani V Journal of industrial microbiology & biotechnology . 2009,第1期

机译：用游离和聚乙烯醇固定的米曲霉中的游离和聚乙烯醇固定的酶法去除鹰嘴豆牛奶中的肠胃气胀性糖
3. Removal of Flatulence-inducing Sugars by Using Free and Polyvinyl Alcohol Immobilized a-Galactosidase from Aspergillus oryzae [J] . Aravind Goud G. Patil, Veerappa H. Mulimani Biotechnology and bioprocess engineering . 2008,第3期

机译：使用游离和聚乙烯醇固定的米曲霉中的游离半乳糖和α-半乳糖苷酶去除肠胃胀气糖
4. Biobleaching Efficiency of Aspergillus oryzae Xylanases Produced by Solid Substrate Fermentation [C] . Judit Szendefy, George Szakacs, Lew Christopher, American Chemical Society National Meeting . 2004

机译：通过固体基质发酵产生的曲霉属植物的生物漂白效率
5. Production of galacto-oligosaccharides from lactose by Aspergillus oryzae beta-galactosidase immobilized on cotton cloth. [D] . Albayrak, Nedim. 2001

机译：通过固定在棉布上的米曲霉β-半乳糖苷酶从乳糖生产半乳糖低聚糖。
6. Synthesis of Oligosaccharides Derived from Lactulose (OsLu) Using Soluble and Immobilized Aspergillus oryzae β-Galactosidase [O] . Alejandra Cardelle-Cobas, Agustin Olano, Gabriela Irazoqui, 2016

机译：可溶性固定化米曲霉β-半乳糖苷酶合成乳果糖（OsLu）寡糖
7. Cooperation between β‐galactosidase and an isoprimeverose‐producing oligoxyloglucan hydrolase is key for xyloglucan degradation in Aspergillus oryzae [O] . Tomohiko Matsuzawa, Masahiro Watanabe, Tomoshi Kameda, 2019

机译：β-半乳糖苷酶和产物寡糖之间的合作是叶绿藻葡萄牙葡萄牙葡萄糖降解的关键

Novel β-galactosynthase–β-mannosynthase dual activity of β-galactosidase from Aspergillus oryzae uncovered using monomer sugar substrates

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