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Flavonol-based fluorescent indicator for determination of β-glucosidase activity

机译:基于黄酮类的荧光指示剂测定β-葡糖苷酶活性

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A novel fluorescent probe, based on the excited-state intramolecular proton transfer (ESIPT) phenomenon, for evaluation of β-glucosidase activity was designed. The synthesis of the probe was optimized. The conditions for the monitoring of enzymatic cleavage of the probe were developed and discussed from the point of view of reaction kinetics and simplicity of the method.
机译:设计了一种新的荧光探针,基于激发状态分子内质子转移(ESIPT)现象,用于评估β-葡糖苷酶活性。优化了探针的合成。从反应动力学和方法的简单性的角度,开发并讨论了监测探针酶切割的条件。

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