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首页> 外文期刊>RSC Advances >Nioplexes encapsulated in supramolecular hybrid biohydrogels as versatile delivery platforms for nucleic acids
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Nioplexes encapsulated in supramolecular hybrid biohydrogels as versatile delivery platforms for nucleic acids

机译：滴管Xes封装在超分子杂交生物氢化合物中，作为核酸的多功能递送平台

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Supramolecular hydrogels based on N-protected phenylalanine (Fmoc–Phe–OH) were used to encapsulate non-ionic surfactant vesicles (niosomes). The niosomes consisted of an amphiphilic lipid mixed with polysorbate-80 and electrostatically complexed with a fluorescently labelled oligodeoxynucleotide (FITC–ODN) as a model nucleic acid derivative. The diffusion properties of the supramolecular hydrogel were conveniently tuned by adding a small amount of κ-carrageenan (≤1% w/v) as a crosslinking agent. Interestingly, neither cationic niosomes nor the biopolymer additive significantly affected the hydrogelation properties of the amino acid-based low molecular weight (LMW) gelator. In vitro drug release experiments from Fmoc–Phe–OH hydrogels containing cationic niosomes were successfully carried out in the absence and in the presence of κ-carrageenan. The niosomal ODN liberation in solution was fitted using Higuchi, Korsmeyer–Peppas and Weibull drug release models, showing the prevalence of diffusion mechanisms in each case. Moreover, the time release was easily prolonged by increasing the concentration of κ-carrageenan. Preliminary transfection studies indicate the suitability of these supramolecular hybrid hydrogels to embed niosomal formulations and, consequently, for being used as tunable delivery vehicles for nucleic acids.
机译：基于N保护苯丙氨酸（FMOC-PHE-OH）的超分子水凝胶用于包封非离子表面活性剂囊泡（Niosomes）。定期组织由与聚山梨醇酯-80混合的两亲性脂质组成，并用荧光标记的寡核酸核苷酸（FITC-ODN）静电络合作为模型核酸衍生物。通过作为交联剂加入少量κ-塞格兰班（≤1％w / v）作为交联剂，方便地调节超分子水凝胶的扩散性能。有趣的是，阳离子定位素和生物聚合物添加剂都没有显着影响氨基酸的低分子量（LMW）胶凝胶的水凝胶化性质。在含有阳离子术中的FMOC-PHE-OH水凝胶中的体外药物释放实验在不存在和存在的情况下成功地进行了κ-角叉菜胶的存在。溶液中的终结ODN释放用Higuchi，Korsmeyer-Peppas和Weibull药物释放模型拟合，显示了每种情况下的扩散机制的普遍性。此外，通过增加κ-角叉菜胶的浓度容易延长时间释放。初步转染研究表明这些超分子杂交水凝胶的适用性嵌入憩室制剂，因此用于用作核酸的可调谐递送载体。

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