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Biological tooth root reconstruction with a scaffold of swine treated dentin matrix

机译：用猪处理牙本质矩阵的生物齿重建

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Treated dentin matrix (TDM) is an ideal scaffolding material with odontogenic ability, which is important for supporting cell growth and regeneration of dental tissue. The source of TDM could be a key point for clinical application in the future. Xenogenic TDM from swine (sTDM) may be an ideal alternative because of its high similarities with human anatomy and structure. However, due to species-specific differences, it remains unclear whether TDM fabrication methods can be extended to sTDM. In the present study, we have optimized the fabrication method to fabricate a sTDM scaffold material with odontogenic ability. Furthermore, dental follicle cells from swine (sDFCs) were combined with sTDM and were implanted into the alveolar fossa of swine to reconstruct the biological tooth root in vivo . The results showed the step-by-step process of demineralization by EDTA was effective and necessary. The treatment times with 17% EDTA, 10% EDTA and 5% EDTA should be at least 15 min, 15 min, and 5 min, respectively. Under the induction of sTDM, the growth and viability of seeding cells were significantly improved. Most importantly, sTDM in vivo could induce and support the regeneration of complete tooth root tissues. The cells within and surrounding the regenerated tissues were positive for green fluorescent protein (GFP), indicating that sDFSCs are responsible for the regenerated tooth root tissues. In conclusion, sTDM, which was fabricated by an optimized method, could be suitable for reconstruction of tooth roots in vivo in swine.
机译：经过处理的牙本质基质（TDM）是具有牙科源性能力的理想脚手架材料，这对于支持细胞生长和牙科组织的再生是重要的。 TDM的来源可能是未来临床应用的关键点。由于其与人解剖结构和结构的高相似性，来自猪（STDM）的异种TDM可能是理想的替代品。但是，由于物种特异性差异，仍不清楚TDM制造方法是否可以扩展到STDM。在本研究中，我们优化了制造方法，以制造具有牙植能力的STDM支架材料。此外，来自猪（SDFC）的牙科卵泡细胞与STDM合并，并植入猪的肺泡窝中，以重建体内生物齿根。结果表明，EDTA的脱矿化的逐步过程是有效且必需的。具有17％EDTA，10％EDTA和5％EDTA的处理时间应至少为15分钟，15分钟和5分钟。在STDM的诱导下，播种细胞的生长和活力显着改善。最重要的是，体内的STDM可以诱导和支持完整的牙根组织的再生。再生组织内和周围的细胞对于绿色荧光蛋白（GFP）是阳性的，表明SDFSCs负责再生齿根组织。总之，通过优化方法制造的STDM可能适用于猪中体内牙根的重建。

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