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首页> 外文期刊>RSC Advances >Silver nanoparticles with different size and shape: equal cytotoxicity, but different antibacterial effects
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Silver nanoparticles with different size and shape: equal cytotoxicity, but different antibacterial effects

机译：具有不同尺寸和形状的银纳米粒子：等于细胞毒性，但不同的抗菌作用

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The influence of silver nanoparticle morphology on the dissolution kinetics in ultrapure water as well as the biological effect on eukaryotic and prokaryotic cells was examined. Silver nanoparticles with different shapes but comparable size and identical surface functionalisation were prepared, i.e. spheres (diameter 40–80 and 120–180 nm; two different samples), platelets (20–60 nm), cubes (140–180 nm), and rods (diameter 80–120 nm, length > 1000 nm). All particles were purified by ultracentrifugation and colloidally stabilized with poly( N -vinyl pyrrolidone) (PVP). Their colloidal dispersion in ultrapure water and cell culture medium was demonstrated by dynamic light scattering. Size, shape, and colloidal stability were analysed by scanning electron microscopy, atomic force microscopy, dynamic light scattering, and differential centrifugal sedimentation. The dissolution in ultrapure water was proportional to the specific surface area of the silver nanoparticles. The averaged release rate for all particle morphologies was 30 ± 13 ng s ~(?1) m ~(?2) in ultrapure water ( T = 25 ± 1 °C; pH 4.8; oxygen saturation 93%), i.e. about 10–20 times larger than the release of silver from a macroscopic silver bar (1 oz), possibly due to the presence of surface defects in the nanoparticulate state. All particles were taken up by human mesenchymal stem cells and were cytotoxic in concentrations of >12.5 μg mL ~(?1) , but there was no significant influence of the particle shape on the cytotoxicity towards the cells. Contrary to that, the toxicity towards bacteria increased with a higher dissolution rate, suggesting that the toxic species against bacteria are dissolved silver ions.
机译：研究了银纳米粒子形态对超纯水溶出动力学的影响以及对真核和原核细胞的生物学作用。制备具有不同形状但相当的表面官能化的银纳米颗粒，即球形（直径40-80和120-180nm;两种不同的样品），血小板（20-60nm），立方体（140-180nm），和棒（直径80-120 nm，长度> 1000nm）。通过超速离心纯化所有颗粒并用聚（N-乙烯基吡咯烷酮）（PVP）胆体稳定。通过动态光散射证明了它们在超纯水和细胞培养基中的胶体分散体。通过扫描电子显微镜，原子力显微镜，动态光散射和差动离心沉降来分析尺寸，形状和胶体稳定性。超纯水中的溶解与银纳米颗粒的比表面积成比例。所有粒子形态的平均释放率为超纯水中的30±13ng s〜（α1）m〜（Δ2）（t = 25±1°C; pH 4.8;氧饱和93％），即约10-来自宏观银棒（1盎司）的银释放的20倍，可能是由于纳米颗粒状态下表面缺陷的存在。所有颗粒都被人间充质干细胞吸收，浓度为>12.5μgml〜（α1），但颗粒形状对细胞的细胞毒性没有显着影响。与此相反，对细菌的毒性随着较高的溶解速率而增加，表明对细菌的有毒物种是溶解的银离子。

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