• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >Potential antiviral activities of camel, bovine, and human lactoperoxidases against hepatitis C virus genotype 4
【24h】

Potential antiviral activities of camel, bovine, and human lactoperoxidases against hepatitis C virus genotype 4

机译：骆驼，牛和人乳酰氧化酶对丙型肝炎病毒基因型4的潜在抗病毒活动4

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Illnesses caused by hepatitis C virus (HCV) represent a major threat in Egypt and worldwide since there are still no efficient protective vaccines against the HCV infection. A large number of patients use camel milk as an alternative medicine to control the HCV infection because of the high cost of the available standard therapy. Milk contains several proteins (including lactoperoxidase, LPO) with a broad spectrum of activity against pathogenic microorganisms and viruses. In this study, the effectiveness of human, camel, and bovine LPOs against HCV genotype 4 was assayed using the RT-nested PCR and real time PCR techniques. Pre-incubation of the HepG2 cells with human, camel, or bovine LPOs which were then infected with HCV failed to block the receptors of the virus on the cell surface. However, direct interaction of HCV with LPO at concentrations of 1.5 mg ml ~(?1) led to a complete neutralization of the viral particles and prevented the HCV entry to the cells. LPOs were also able to inhibit virus amplification in infected HepG2 cells at concentrations of 1.25 and 1.5 mg ml ~(?1) with a relative activity of 100%. The highest anti-infectivity was demonstrated by the camel LPO, which showed a neutralizing activity on HCV particles and inhibition of virus amplification at a concentration of 1.0 mg ml ~(?1) with the relative activity of 100%. This is first report that evaluates the effectiveness of LPO in controlling HCV infectivity.
机译：丙型肝炎病毒（HCV）引起的疾病（HCV）代表了埃及和全球的主要威胁，因为仍然没有有效的保护性疫苗对HCV感染。由于可用标准治疗的高成本，大量患者用骆驼牛奶作为替代药物来控制HCV感染。牛奶含有几种蛋白质（包括乳酰氧化酶，LPO），具有广泛的致病微生物和病毒的活性。在该研究中，使用RT-NETED PCR和实时PCR技术测定人，骆驼和牛LPO对HCV基因型4的有效性。然后用HCV感染的HEPG2细胞与人，骆驼或牛LPO的预孵育未能阻断病毒在细胞表面上的受体。然而，HCV与LPO的直接相互作用在1.5mg mL〜（α1）的浓度下导致病毒颗粒完全中和，并防止HCV进入细胞。 LPOS还能够在1.25和1.5mg ml〜（α1）的浓度下抑制感染的HepG2细胞中的病毒扩增，其相对活性为100％。骆驼LPO证明了最高的抗感染性，其在HCV颗粒上显示了中和活性，并以1.0mg ml〜（α1）的浓度为100％的浓度抑制病毒扩增。这是第一个报告，评估LPO控制HCV感染性的有效性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号