• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >Potential antiviral activities of camel, bovine, and human lactoperoxidases against hepatitis C virus genotype 4
【24h】

Potential antiviral activities of camel, bovine, and human lactoperoxidases against hepatitis C virus genotype 4

机译：骆驼，牛和人乳过氧化物酶对丙型肝炎病毒基因型4的潜在抗病毒活性

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Illnesses caused by hepatitis C virus (HCV) represent a major threat in Egypt and worldwide since there are still no efficient protective vaccines against the HCV infection. A large number of patients use camel milk as an alternative medicine to control the HCV infection because of the high cost of the available standard therapy. Milk contains several proteins (including lactoperoxidase, LPO) with a broad spectrum of activity against pathogenic microorganisms and viruses. In this study, the effectiveness of human, camel, and bovine LPOs against HCV genotype 4 was assayed using the RT-nested PCR and real time PCR techniques. Pre-incubation of the HepG2 cells with human, camel, or bovine LPOs which were then infected with HCV failed to block the receptors of the virus on the cell surface. However, direct interaction of HCV with LPO at concentrations of 1.5 mg ml(-1) led to a complete neutralization of the viral particles and prevented the HCV entry to the cells. LPOs were also able to inhibit virus amplification in infected HepG2 cells at concentrations of 1.25 and 1.5 mg ml(-1) with a relative activity of 100%. The highest anti-infectivity was demonstrated by the camel LPO, which showed a neutralizing activity on HCV particles and inhibition of virus amplification at a concentration of 1.0 mg ml(-1) with the relative activity of 100%. This is first report that evaluates the effectiveness of LPO in controlling HCV infectivity.
机译：由丙型肝炎病毒（HCV）引起的疾病是埃及乃至全世界的主要威胁，因为仍然没有针对HCV感染的有效保护性疫苗。由于可用的标准疗法成本高昂，大量患者使用骆驼奶作为控制HCV感染的替代药物。牛奶含有多种蛋白质（包括乳过氧化物酶，LPO），具有广泛的抗病原微生物和病毒的活性。在这项研究中，使用RT巢式PCR和实时PCR技术测定了人，骆驼和牛LPO对HCV基因型4的有效性。将HepG2细胞与人，骆驼或牛LPO预先孵育，然后再感染HCV，无法阻止病毒在细胞表面的受体。然而，浓度为1.5 mg ml（-1）的HCV与LPO的直接相互作用导致病毒颗粒的完全中和并阻止HCV进入细胞。 LPO还能够以1.25和1.5 mg ml（-1）的浓度抑制感染的HepG2细胞中的病毒扩增，相对活性为100％。骆驼LPO证明了最高的抗感染性，它对HCV颗粒具有中和活性，在1.0 mg ml（-1）的浓度下具有100％的相对活性，可抑制病毒的扩增。这是第一份评估LPO在控制HCV感染性中有效性的报告。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号