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首页> 外文期刊>RSC Advances >Amino acid-based poly(ester amide) nanofibers for tailored enzymatic degradation prepared by miniemulsion-electrospinning
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Amino acid-based poly(ester amide) nanofibers for tailored enzymatic degradation prepared by miniemulsion-electrospinning

机译：基于氨基酸的聚（酯酰胺）纳米纤维，用于制备小型乳液 - 静电纺丝制备的定制酶促降解

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摘要

Novel enzyme loaded scaffolds with enzyme-responsive degradable properties for drug delivery are prepared by an original inverse-miniemulsion electrospinning method. Miniemulsions with aqueous nanodroplets containing different enzymes, i.e. lipase or α-chymotrypsin, and a fluorophore are electrospun with a solution of poly(ester amide) and polycaprolactone to fabricate multicompartment nanofibers. The poly(ester amide) contains the two essential amino acids phenylalanine and leucine that promote low cytotoxicity degradation products and makes them suitable for the preparation of drug delivery devices for the biomedical field. The activity of the loaded enzymes in different conditions and a sustained degradation of fibers mechanism with an approximate 20% weight loss within one month are observed. Locating enzymes in degradation medium accelerated the degradation until complete scaffold destruction in less than 5 days. In all cases, a nearly complete release of the loaded fluorophore (from 80% and upwards) was achieved before the complete degradation of fibers occurred, suggesting that the nanofibers are suitable as self-triggered drug release systems with sustained mechanical integrity and a flexible range of degradation rates.
机译：通过原始的逆型乳液电刺纺丝方法制备具有酶响应性可降解性的酶响应性可降解性能的新型酶的支架。具有含有不同酶的含水纳米液，即脂肪酶或α-胰蛋白酶，以及荧光团，具有聚（酯酰胺）和聚己内酯的聚己内酯溶液，以制造多分量纳米纤维，含有不同酶的微量乳液。聚（酯酰胺）含有两种必需的氨基酸苯丙氨酸和亮氨酸，可促进低细胞毒性降解产物，并使它们适用于制备生物医学领域的药物递送装置。观察到在不同条件下加载酶的活性和纤维机制的持续降解，在一个月内具有近似20％的体重减轻。降解培养基中的定位酶加速降解直至在少于5天内完成支架破坏。在所有情况下，在发生纤维的完全降解之前，在发生纤维的完全降解之前实现了近乎完全释放的荧光团（从80％和向上），这表明纳米纤维适合作为自动触发的药物释放系统，具有持续的机械完整性和柔性范围退化率。

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