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首页> 外文期刊>RSC Advances >Amino acid-based poly(ester amide) nanofibers for tailored enzymatic degradation prepared by miniemulsion-electrospinning
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Amino acid-based poly(ester amide) nanofibers for tailored enzymatic degradation prepared by miniemulsion-electrospinning

机译：通过微乳液静电纺丝制备的用于定制酶促降解的基于氨基酸的聚（酯酰胺）纳米纤维

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摘要

Novel enzyme loaded scaffolds with enzyme-responsive degradable properties for drug delivery are prepared by an original inverse-miniemulsion electrospinning method. Miniemulsions with aqueous nanodroplets containing different enzymes, i.e. lipase or alpha-chymotrypsin, and a fluorophore are electrospun with a solution of poly(ester amide) and polycaprolactone to fabricate multicompartment nanofibers. The poly(ester amide) contains the two essential amino acids phenylalanine and leucine that promote low cytotoxicity degradation products and makes them suitable for the preparation of drug delivery devices for the biomedical field. The activity of the loaded enzymes in different conditions and a sustained degradation of fibers mechanism with an approximate 20% weight loss within one month are observed. Locating enzymes in degradation medium accelerated the degradation until complete scaffold destruction in less than 5 days. In all cases, a nearly complete release of the loaded fluorophore (from 80% and upwards) was achieved before the complete degradation of fibers occurred, suggesting that the nanofibers are suitable as self-triggered drug release systems with sustained mechanical integrity and a flexible range of degradation rates.
机译：通过原始的逆分子乳液静电纺丝法制备具有用于药物递送的酶响应可降解特性的新型载酶支架。将含有不同酶（即脂肪酶或α-胰凝乳蛋白酶）和荧光团的水性纳米液滴的细乳液与聚（酯酰胺）和聚己内酯溶液电纺丝，制成多隔室的纳米纤维。聚（酰胺酰胺）包含两个必需氨基酸，苯丙氨酸和亮氨酸，可促进低细胞毒性降解产物，并使其适合用于生物医学领域的药物递送装置的制备。观察到负载酶在不同条件下的活性以及纤维机理的持续降解，在一个月内重量减少了约20％。将酶置于降解培养基中可加速降解，直到在不到5天的时间内完全破坏支架。在所有情况下，在发生纤维完全降解之前，已实现了负载的荧光团的几乎完全释放（从80％起），这表明纳米纤维适合作为具有机械机械稳定性和灵活范围的自触发药物释放系统降解率。

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