Click chemistry facilitates direct labelling and super-resolution imaging of nucleic acids and proteins

Anika Raulf; Christoph K. Spahn; Patrick J. M. Zessin; Kieran Finan; Stefan Bernhardt; Alexander Heckel; Mike Heilemann

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Click chemistry facilitates direct labelling and super-resolution imaging of nucleic acids and proteins

机译：点击化学促进核酸和蛋白质的直接标记和超分辨率成像

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We demonstrate high-density labelling of cellular DNA and RNA using click chemistry and perform confocal and super-resolution microscopy. We visualize the crescent and ring-like structure of densely packed RNA in nucleoli. We further demonstrate click chemistry with unnatural amino acids for super-resolution imaging of outer-membrane proteins of E. coli .

机译：我们用咔哒化学证明了蜂窝DNA和RNA的高密度标记，并进行了共聚焦和超分辨率显微镜。我们在核仁中可视化Crescent和Ring样的浓密填充RNA的结构。我们进一步展示了单击化学与非天然氨基酸，用于大肠杆菌外膜蛋白的超分辨率成像。

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《RSC Advances》 |2014年第57期|共5页
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Anika Raulf; Christoph K. Spahn; Patrick J. M. Zessin; Kieran Finan; Stefan Bernhardt; Alexander Heckel; Mike Heilemann;
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1. Site-Directed Spin-Labeling of Nucleic Acids by Click Chemistry: Detection of Abasic Sites in Duplex DNA by EPR Spectroscopy [J] . Ulla Jakobsen, rnSandip A. Shelke, rnStefan Vogel, Journal of the American Chemical Society . 2010,第30期

机译：单击化学的核酸定点自旋标记：通过EPR光谱法检测双链DNA中的碱性位点
2. Site-Directed Spin-Labeling of Nucleic Acids by Click Chemistry: Detection of Abasic Sites in Duplex DNA by EPR Spectroscopy [J] . Ulla Jakobsen Journal of the American Chemical Society . 2010,第30期

机译：单击化学的核酸定点自旋标记：通过EPR光谱法检测双链DNA中的碱性位点
3. Combined Use of Unnatural Amino Acids Enables Dual-Color Super-Resolution Imaging of Proteins via Click Chemistry [J] . Saal Kim-A., Richter Frank, Rehling Peter, ACS nano . 2018,第12期

机译：联合使用非天然氨基酸可以通过点击化学来实现蛋白质的双色超分辨率成像
4. Investigating conformational changes of protein-nucleic acid and protein-ligand-nucleic acid complexes by tandem mass spectrometry [C] . Kevin B. Turner, Daniele Fabris ASMS Conference on Mass Spectrometry and Allied Topics . 2008

机译：通过串联质谱研究蛋白质 - 核酸和蛋白质 - 配体 - 核酸复合物的构象变化
5. Motions and conformations of nucleic acids studied using site-directed spin labeling. [D] . Grant, Gian Paola Gacho. 2009

机译：使用定点旋转标记研究的核酸的运动和构象。
6. Super-Resolution Imaging of Plasma Membrane Proteins with Click Chemistry [O] . Pablo Mateos-Gil, Sebastian Letschert, Sören Doose, 2016

机译：单击化学对血浆膜蛋白的超分辨率成像
7. Super-resolution imaging of plasma membrane proteins with click chemistry [O] . Pablo Mateos-Gil, Sebastian Letschert, Soeren Doose, 2016

机译：点击化学对质膜蛋白进行超分辨率成像

Click chemistry facilitates direct labelling and super-resolution imaging of nucleic acids and proteins

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