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首页> 外文期刊>Scientific reports. >Simultaneous co-localized super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for the life sciences
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Simultaneous co-localized super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for the life sciences

机译:同时共定分辨率荧光显微镜和原子力显微镜:综合SIM和AFM平台的生命科学

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Correlating data from different microscopy techniques holds the potential to discover new facets of signaling events in cellular biology. Here we report for the first time a hardware set-up capable of achieving simultaneous co-localized imaging of spatially correlated far-field super-resolution fluorescence microscopy and atomic force microscopy, a feat only obtained until now by fluorescence microscopy set-ups with spatial resolution restricted by the Abbe diffraction limit. We detail system integration and demonstrate system performance using sub-resolution fluorescent beads and applied to a test sample consisting of human bone osteosarcoma epithelial cells, with plasma membrane transporter 1 (MCT1) tagged with an enhanced green fluorescent protein (EGFP) at the N-terminal.
机译:从不同显微镜技术相关数据具有发现蜂窝生物学中信令事件的新方面的可能性。在这里,我们首次报告一种硬件设置能够实现空间相关的远场超分辨率荧光显微镜和原子力显微镜的同时共同局部成像,仅通过具有空间的荧光显微镜设置才能获得壮举。由ABBE衍射限制限制的分辨率。我们使用子分辨率荧光珠子详细说明系统集成,并展示系统性能,并应用于由人骨骨肉瘤上皮细胞组成的测试样品,血浆膜转运蛋白1(MCT1)标记为在N-中用增强的绿色荧光蛋白(EGFP)。终端。

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