...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of bacteriology >Genomic Differences between Listeria monocytogenes EGDe Isolates Reveal Crucial Roles for SigB and Wall Rhamnosylation in Biofilm Formation
【24h】

Genomic Differences between Listeria monocytogenes EGDe Isolates Reveal Crucial Roles for SigB and Wall Rhamnosylation in Biofilm Formation

机译：Histeria单核细胞增生的基因组差异Egde分离物揭示了生物膜形成中Sigb和Wall rhamnosation的至关重要作用

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Listeria monocytogenes is a Gram-positive firmicute that causes foodborne infections, in part due to its ability to use multiple strategies, including biofilm formation, to survive adverse growth conditions. As a potential way to screen for genes required for biofilm formation, we harnessed the ability of bacteria to accumulate mutations in the genome over time, diverging the properties of seemingly identical strains. By sequencing the genomes of four laboratory reference strains of the commonly used L. monocytogenes EGDe, we showed that each isolate contains single nucleotide polymorphisms (SNPs) compared with the reference genome. We discovered that two SNPs, contained in two independent genes within one of the isolates, impacted biofilm formation. Using bacterial genetics and phenotypic assays, we confirmed that rsbU and rmlA influence biofilm formation. RsbU is the upstream regulator of the alternative sigma factor SigB, and mutation of either rsbU or sigB increased biofilm formation. In contrast, deletion of rmlA , which encodes the first enzyme for TDP-l-rhamnose biosynthesis, resulted in a reduction in the amount of biofilm formed. Further analysis of biofilm formation in a strain that still produces TDP-l-rhamnose but which cannot decorate the wall teichoic acid with rhamnose ( rmlT mutant) showed that it is the decorated wall teichoic acid that is required for adhesion of the cells to surfaces. Together, these data uncover novel routes by which biofilm formation by L. monocytogenes can be impacted. IMPORTANCE Biofilms are an important mode of growth in many settings. Here, we looked at small differences in the genomes of the bacterium Listeria monocytogenes isolate EGDe and used them to find out how biofilms form. This important fundamental information may help new treatments to be developed and also highlights the fact that isolates of the same identity often diverge.
机译：Listeria单核细胞增粒是一种革兰氏阳性的成功，导致食源性感染，部分是由于其使用多种策略，包括生物膜形成，以存活不利生长条件。作为筛选生物膜形成所需基因的潜在方法，我们利用细菌在基因组中积聚的能力随着时间的推移，散发出看似相同的菌株的性质。通过测序常用的L.单核细胞增强EGDE的四种实验室参考菌株的基因组，我们表明，与参考基因组相比，每个分离物含有单核苷酸多态性（SNP）。我们发现两个SNP，其中包含两个分离物中的两个独立基因，受影响的生物膜形成。使用细菌遗传学和表型测定，我们证实RSBU和RMLA影响生物膜形成。 RSBU是替代Sigma系数SigB的上游调节器，RSBU或SIGB的突变增加了生物膜形成。相反，对TDP-L-rhamnose生物合成的第一酶进行编码的RMLA缺失导致形成的生物膜的量减少。进一步分析仍然产生TDP-L-rhamnose的菌株中的生物膜形成，但不能用鼻窦（RMLT突变体）装饰壁吡酰酸，表明它是装饰壁噻吩，其需要将细胞与表面粘附到表面。这些数据揭示了L.单核细胞元的生物膜形成的新型途径可能会受到影响。重要性生物膜是许多环境中的一个重要增长方式。在这里，我们看着细菌李斯特菌李单核细胞增生的基因组分离出EGDE的小差异，并用它们来了解生物膜的形式。这一重要的基本信息可能有助于开发新的治疗，并突出了同一身份的分离常见的事实。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号