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首页> 外文会议>International Conference on Environmental, Industrial and Applied Microbiology >Differences between clinical and food isolates of Listeria monocytogenes in biofilm formation
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Differences between clinical and food isolates of Listeria monocytogenes in biofilm formation

机译：生物膜形成中李斯特菌单核细胞植物临床和食品分离物的差异

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Pathogenic microorganisms such as Listeria monocytogenes are known to form biofilms. The risk of contamination of food products in the food industry and growth on medical devices in the clinical environment is a cause of concern. The objectives of this work were to examine and compare biofilm formation by food and clinical isolates of L. monocytogenes. A total of 376 L. monocytogenes strains, 258 isolated from various food products and 118 clinical isolates, were investigated with respect to their ability to form biofilms in 96-wells microtiter plates, at 4 °C during 5 days and at 37 °C during 24h. Statistically significant differences (p < 0.01) were found between biofilm formation at 4 °C or at 37 °C. At 4 °C, statistically significant differences between clinical and food isolates were observed (p < 0.01). Although being classified as weak producers, more food isolates produced biofilms than clinical isolates. Statistically significant differences (p < 0.01) between clinical and food isolates were also observed at 37 °C. However, at this temperature, more clinical isolates produced biofilms than food isolates; 4% and 0.4% of the clinical and food isolates investigated, respectively, were classified as strong biofilm formers. Biofilm production was demonstrated to differ between clinical and food isolates, but these differences were temperature dependent.
机译：已知毒性微生物，例如李斯特菌单核细胞元形成生物膜。临床环境中食品工业中食品污染和生长的风险是令人关切的原因。这项工作的目标是通过食物和L.单核细胞增生的食物和临床分离株进行检查和比较生物膜形成。在5天和37℃下，相对于它们在96孔微量滴定板中形成生物膜的能力，研究了从各种食品和118个临床分离物中分离的376升单核细胞增生菌株258。 24h。在4℃或37℃下在生物膜形成之间发现统计学上显着的差异（P <0.01）。在4℃下，观察到临床和食物分离株之间的统计学显着差异（P <0.01）。虽然被归类为弱生生产者，但更多的食物分离物产生的生物膜比临床分离物。在37℃下也观察到临床和食品分离物之间的统计学显着差异（P <0.01）。然而，在这种温度下，更多的临床分离物产生比食品分离物的生物膜;分别研究了4％和0.4％的临床和食物分离物，分为强生物膜成型剂。生物膜生产被证明是临床和食品分离物之间的不同，但这些差异是温度依赖性。

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