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The application of 1H NMR to explore the taste difference caused by taste‐active metabolites of different Chinese sauce‐stewed beef

机译:1H NMR探讨不同中国酱油炖牛肉味道活性代谢产物引起的味道差异

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In this study, we developed a method for the quantification of taste‐active metabolites of Chinese commercial sauce‐stewed beef by using 1H NMR spectroscopy coupled with multivariate data analysis. This method was applied to analyze the taste difference which caused by taste‐active metabolites of different Chinese sauce‐stewed beef. Beef samples demonstrated to consist of 25 metabolites, including amino acids, sugars, organic acids, nucleic aides, and their derivatives. PC1 and PC2 explained a total of 85.1 and 13.1% of variables, respectively. Metabolites such as isoleucine, histidine, glutamate, pyroglutamate, sucrose, lactate, creatine, carnitine, and creatinine were kept at a higher levels compared with other metabolites in the four products. Sensory evaluation was also done to help analyze the taste difference. This microcosmic approach of using high‐resolution NMR spectrometry to analyze beef products has rarely been reported. This work established a feasible method to distinguish the taste difference of different Chinese sauce‐stewed beef.
机译:在这项研究中,我们通过使用1H NMR光谱与多变量数据分析耦合的1H NMR光谱来制定一种定量中国商业酱油炖牛肉的味道活性代谢物的方法。应用该方法以分析由不同中国酱油炖牛肉的味道活性代谢物引起的味道差异。牛肉样品证明由25种代谢物组成,包括氨基酸,糖,有机酸,核助剂及其衍生物。 PC1和PC2分别解释了总共85.1和13.1%的变量。与四种产物中的其他代谢物相比,与四种产品中的其他代谢产物相比,等代谢产物如异氨氨酸,组氨酸,谷氨酸,吡酰胺,蔗糖,乳酸,肌酸,肉碱和肌酐。还进行了感官评估,以帮助分析味道差异。这种使用高分辨率NMR光谱法分析牛肉产品的微观方法已经很少报道。这项工作建立了一种可行的方法,以区分不同中国酱油炖牛肉的味道差异。

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