Physicochemical and rheological characterization of pectin‐rich polysaccharides from Gardenia jasminoides J. Ellis flower

Qi Chen; Gang Xue; Qinxue Ni; Yan Wang; Qianxin Gao; Youzuo Zhang; Guangzhi Xu

摘要

Gardenia (Gardenia jasminoides J. Ellis) is regarded as an edible medicine plant in China. Here, gardenia flower polysaccharide fraction (GFPF) was extracted by water at 90°C and its chemical composition, rheological properties, and antioxidant activities of GFPF were investigated. The GFPF extraction yield was 18.04?±?1.81% (W/W) and mainly comprised neutral sugars (46.83?±?3.14%), uronic acid (35.21?±?0.17%), protein (1.63?±?0.34%), and total phenol (9.49?±?0.08 mgGAE/g). Galacturonic acid (41.05?±?0.59%) was the main monosaccharide, and galactose, glucose, arabinose, rhamnose, xylose, mannose, and glucuronic acid were also detected in GFPF. Its degree of esterification was 32.76?±?1.52%. FT‐IR spectra analysis showed a similar absorption pattern between GFPF and pectin from apple. The results suggested that GFPF was low methoxy pectin. Thermogravimetric analysis and zeta potential analysis indicated that the pectin was stable under high temperature and alkaline condition. Steady rheology showed that the GFPF dispersion was a shear thinned pseudoplastic fluid with high apparent viscosities at concentration above 2%. The degree of pseudoplasticity of the solutions increased with the concentrations increased and the temperatures decreased. DPPH and ABTS free radical scavenging assay indicated that GFPF had relatively high antioxidant activity. The results showed that gardenia flower was rich in pectin polysaccharides with low methoxy pectin. It had high apparent viscosities at concentration above 2% and had good antioxidant activity. The data suggested that GFPF can be a new resource of low methoxy pectin with potential application as thicker or gelling agents in food industry.

机译：Gardenia（Gardenia Jasminoides J. Ellis）被认为是中国的可食用药厂。这里，栀子花多糖馏分（GFPF）被水在90℃下萃取，并研究了其化学成分，流变性能和GFPF的抗氧化活性。 GFPF萃取产率为18.04？±1.81％（w / w），主要包含中性糖（46.83？±3.14％），助核（35.21〜±0.17％），蛋白质（1.63？±0.34％），和总苯酚（9.49？±0.08 mggae / g）。半糖（41.05→0.59％）是主要的单糖，半乳糖，葡萄糖，阿拉伯糖，鼠李糖，木糖，甘露糖和血糖醛酸也被检测到GFPF中。其酯化程度为32.76？±1.52％。 FT-IR光谱分析显示GFPF与苹果果胶之间的类似吸收模式。结果表明GFPF是低甲氧基果胶。热重分析和Zeta电位分析表明果胶在高温和碱性条件下稳定。稳态流变学表明，GFPF分散体是剪切薄的假塑料流体，浓度高于2％的浓度高的表观粘度。溶液随浓度的增加而增加的溶液的假塑性程度增加，温度降低。 DPPH和ABTS自由基清除测定表明GFPF具有相对高的抗氧化活性。结果表明，栀子花富含果胶多糖，具有低甲氧基果胶。它具有高于2％的浓度高的表观粘度，并且具有良好的抗氧化活性。该数据表明，GFPF可以是低甲氧基果胶的新资源，其潜在应用是食品工业中较厚或胶凝剂。

Physicochemical and rheological characterization of pectin‐rich polysaccharides from Gardenia jasminoides J. Ellis flower

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