Antirrhinum majus (AM) has attracted attention as a rich source of phytochemicals, which are beneficial for human health. However, the anti‐inflammatory effects of AM have not been studied scientifically. Therefore, we investigated the antioxidative properties and anti‐inflammatory effects of AM extract (AME) in lipopolysaccharide (LPS)‐stimulated RAW 264.7 macrophages. AME showed high radical‐scavenging ability. Viability of RAW 264.7 cells was not significantly altered by AME at the concentrations of 0–300?μg/ml. LPS‐induced nitric oxide (NO) production was decreased by treatment with 0–300?μg/ml AME in a concentration‐dependent manner. AME pretreatment significantly inhibited the protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase‐2 (COX‐2) in a concentration‐dependent manner. AME also considerably inhibited the mRNA and protein expression of inflammatory cytokines, such as tumor necrosis factor‐a (TNF‐α), interleukin‐1 β (IL‐1β), and interleukin‐6 (IL‐6). These findings provide a foundation for further studies and use of AM in nutraceuticals.
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机译：Antirrhinum Majus（AM）吸引了作为丰富的植物化学来源，这对人类健康有益。然而，尚未科学地研究了AM的抗炎作用。因此，我们研究了脂多糖（LPS） - 刺激的原始264.7巨噬细胞中AM提取物（AME）的抗氧化性能和抗炎作用。 AME表现出高的激进扫荡能力。 ame在0-300Ω×μg/ ml的浓度下不会显着改变原始264.7细胞的可活力。通过以浓度依赖性方式处理0-300Ωμg/ ml ame，通过用0-300×μg/ ml ame治疗来降低LPS诱导的一氧化氮（NO）产生。 AME预处理以浓度依赖性方式显着抑制诱导型一氧化氮合酶（InOS）和环氧化酶-2（COX-2）的蛋白质表达。 AME也大大抑制了炎性细胞因子的mRNA和蛋白表达，例如肿瘤坏死因子-A（TNF-α），白细胞介素-1β（IL-1β）和白细胞介素-6（IL-6）。这些调查结果为进一步研究和使用营养制品提供了基础。
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