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>Application of antagonist Bacillus amyloliquefaciens NCPSJ7 against Botrytis cinerea in postharvest Red Globe grapes
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Application of antagonist Bacillus amyloliquefaciens NCPSJ7 against Botrytis cinerea in postharvest Red Globe grapes
We investigated the effects and possible mechanisms of Bacillus amyloliquefaciens NCPSJ7 against the gray mold caused by Botrytis cinerea in the postharvest Red Globe grapes. The disease incidence, lesion diameter, decay index, and some resistance‐related enzymes were evaluated. The antioxidant capacity of grape treated with 1?×?104?CFU/ml B.?cinerea alone and combined with 1?×?107?CFU/ml NCPSJ7 was also determined. The results showed that NCPSJ7?+?B.?cinerea reduced the disease incidence, lesion diameter, and decay index of postharvest grapes and enhanced the activities of polyphenol oxidase, peroxidase, chitinase, and β‐1,3‐glucanase during different storage periods. Furthermore, the oxidative resistance, demonstrated by an escalating trend in the total phenolic content, DPPH free radical clearance rate, reducing power, and superoxide anion clearance rate after lesion presence, was improved. However, NCPSJ7 showed an inhibitory effect on gray mold, but resulted in the reduced antioxidant capacity in the grapes.
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机译：我们调查了芽孢杆菌淀粉醇氨基唑米加西NCPSJ7对由POTRYTIS Cinerea引起的Postharvest Red Grome葡萄引起的灰霉模具的影响和可能的机制。评估疾病发病率，病变直径，衰减指数和一些抗性相关酶。用1××104×104℃处理葡萄的抗氧化能力，单独使用1〜×107〜1〜×107？CFU / mL NCPSJ7。结果表明，NCPSJ7？+ B.?cinerea降低了前除葡萄葡萄的疾病发病率，病变直径和衰减指数，并增强了多酚氧化酶，过氧化物酶，丁蛋白，β-1,3-葡聚糖酶的活性在不同的储存期间。此外，通过酚醛含量，DPPH自由基间隙率，降低功率和过失存在后的超氧化物阴离子间隙率的升高趋势证明了氧化抗性。然而，NCPSJ7对灰霉模具表现出抑制作用，但导致葡萄中的抗氧化能力降低。
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