...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Stem cells international >Expression Profile of Long Noncoding RNAs and Circular RNAs in Mouse C3H10T1/2 Mesenchymal Stem Cells Undergoing Myogenic and Cardiomyogenic Differentiation
【24h】

Expression Profile of Long Noncoding RNAs and Circular RNAs in Mouse C3H10T1/2 Mesenchymal Stem Cells Undergoing Myogenic and Cardiomyogenic Differentiation

机译：在肌原遗传学和心肌囊性分化的小鼠C3H10T1 / 2间充质干细胞中长度非沉积RNA和圆形RNA的表达谱

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background . Currently, a heterogeneous category of noncoding RNAs (ncRNA) that directly regulate the expression or function of protein-coding genes is shown to have an effect on the fate decision of stem cells. However, the detailed regulatory roles of ncRNAs in myogenic and cardiomyogenic differentiation of mouse C3H10T1/2 mesenchymal stem cells (MSCs) are far from clear. Methods . In this study, 5-azacytidine- (5-AZA-) treated C3H10T1/2 cells were differentiated into myocyte-like and cardiomyocyte-like cells. Next, ncRNA associated with myogenic and cardiomyogenic differentiation was identified using high-throughput RNA sequencing (RNA-seq) data. Bioinformatics analysis was conducted to identify the differentially expressed ncRNAs and the related signaling pathways. Results . Myotube-like structure was formed after 5-AZA treatment of C3H10T1/2 cells. In addition, myogenic and cardiomyogenic differentiation-related genes like GATA4 , cTnt , MyoD , and Desmin were upregulated significantly after the 5-AZA treatment. Totally, 1538 differentially expressed lncRNAs and 3398 differentially expressed mRNAs were identified, including 1175 upregulated and 363 downregulated lncRNAs and 2429 upregulated and 969 downregulated mRNAs. In addition, 46 differentially expressed circRNAs were identified, including 25 upregulated and 21 downregulated circRNAs. Moreover, the differentially expressed mRNAs were enriched into 5 significant pathways, including those for focal adhesion, ECM-receptor interaction, PI3K-AKT signaling pathway, PPAR signaling pathway, and Tyrosine metabolism. Conclusions . A systematic view of the expression of ncRNAs in myogenic and cardiomyogenic differentiation of MSCs was provided in the study.
机译：背景 。目前，将直接调节蛋白质编码基因的表达或功能的异质类别的非致rNA（nCRNA）进行了对干细胞的命运决策的影响。然而，NCRNA在小鼠C3H10T1 / 2间充质干细胞（MSCs）中的肌瘤和心肌细胞分化的详细调节作用远非澄清。方法 。在本研究中，将5-氮杂胞苷 - （5-AZA-）处理的C3H10T1 / 2细胞分化成肌细胞样和心肌细胞样细胞。接下来，使用高通量RNA测序（RNA-SEQ）数据鉴定与肌原素和心肌发生分化相关的NCRNA。进行生物信息学分析以鉴定差异表达的NCRNA和相关信号通路。结果 。在5-AZA处理C3H10T1 / 2细胞后形成肌管状结构。此外，在5-AZA处理后显着上调肌原素和心肌细胞分化相关基因，如GATA4，CTNT，MOSOD和DESMIN。完全，鉴定了1538次差异表达的LNCRNA和3398次差异表达的MRNA，包括1175上调和363下调的LNCRNA和2429上调和969个下调MRNA。此外，鉴定了46个差异表达的CircrNA，包括25个上调和21个下调的Circrna。此外，将差异表达的MRNA富集成5种显着的途径，包括局灶性粘附，ECM-受体相互作用，PI3K-AKT信号传导途径，PPAR信号通路和酪氨酸代谢的那些。结论。研究中，在研究中提供了NCRNA表达的系统视图。在该研究中提供了MSCs的肌原和心肌细胞分化。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号