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首页> 外文期刊>Infection and immunity >Hepatic tissue culture model for study of host-parasite interactions in alveolar echinococcosis.
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Hepatic tissue culture model for study of host-parasite interactions in alveolar echinococcosis.

机译：肺炎术中寄生虫寄生虫相互作用研究的肝组织培养模型。

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摘要

An in vitro model for growth and differentiation of the metacestode tissue of the tapeworm Echinococcus multilocularis is described. This model simulates the organotropism of the parasite toward the liver of the intermediate host. In the presence of collagen-embedded primary hepatocytes from rats and humans, which can be kept in culture for 2 to 3 months, the parasitic vesicles grew by exogenous budding and multiplied about 12-fold within 3 weeks. In contrast, without the hepatocytes, the metacestodes rapidly degenerated. Development of protoscolices was seen only in the presence of rat hepatocytes but not in coculture of the metacestodes with hepatocytes of human origin, thus reflecting the in vivo situation during infection of rodents and in alveolar echinococcosis in humans. The experiments indicated that growth of the metacestodes and development of protoscolices depended on soluble low-molecular-weight factors released by the hepatocytes. The in vitro-grown metacestodes did not differ morphologically from the larvae found in infected intermediate hosts, and their infectivity was completely maintained. This report describes the first in vitro model of alveolar echinococcosis and will be the basis for future studies on host-parasite interactions of this important zoonosis.
机译：描述了绦虫棘波能量障碍组织的生长和分化的体外模型。该模型模拟寄生虫的有机脂肪朝向中间宿主的肝脏。在来自大鼠和人类的胶原嵌入的初级肝细胞存在下，可以在培养物中保持2至3个月，寄生囊泡在外源芽中增长，并在3周内乘以约12倍。相比之下，没有肝细胞，Metacestodes迅速退化。在大鼠肝细胞的存在下仅在大鼠肝细胞存在下观察到原因的发展，但不在人类源性肝细胞的肝细胞的培养中，从而反映了在啮齿动物感染和人类肺泡的肺炎病中的体内情况。该实验表明，肝细胞释放的可溶性低分子量因子，核心溶解度的生长和原子溶解的生长。从感染的中间宿主中发现的幼虫，体外生长的金属纤饼没有与幼虫不同的不同之处，并且完全保持其感染性。本报告描述了肺泡棘球刺症的第一个体外模型，并将是未来研究这一重要的动物病的寄生虫寄生虫相互作用的基础。

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