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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Role of Calretinin Immunohistochemical Stain in Evaluation of Hirschsprung’s Disease
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Role of Calretinin Immunohistochemical Stain in Evaluation of Hirschsprung’s Disease

机译：Calretinin免疫组化染色在Hirschsprung病评价中的作用

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Hirschsprung’s Disease (HD) is a common congenital disorder characterised by absence of enteric neurons along a variable length of large bowel involving a segment of the rectum and the adjacent proximal bowel.The definitive diagnosis is based on histopathological study of rectal seromuscular biopsies.Calretinin Immunohistochemistry (IHC) staining adds to the diagnostic yield as it allows better appreciation of the mature and immature ganglion cells including the segments with paucity of ganglion cells.Aim: To assess the usefulness of calretinin IHC in the diagnosis of HD as an add-on technique to routine Haematoxylin and Eosin staining.Materials and Methods: This was a prospective study carried out in the Department of Pathology, SCB Medical College, Cuttack during the period from August 2016 to July 2018.Full thickness rectal biopsy specimens were processed and subjected to routine Hematoxylin and Eosin Stain (H&E) staining and Calretinin IHC staining.A thorough search of ganglion cells was made in both types of stained slides.The observations were tabulated and compared for diagnostic accuracy.Statistical analysis was done using Statistical Packages for Social Sciences (SPSS) Inc, Chicago IL version 15.0 windows.Through, ANOVA and Chi-square test.Results: Calretinin IHC provided superior diagnostic yield compared to routine H&E staining.A total number of 45 cases were studied.Sensitivity of 95.4% and specificity of 55.5% were obtained.It also presented higher accuracy values, than H&E (p=0.49).Conclusion: Calretinin is a superior immunohistochemical staining method that serves as a reliable additional diagnostic tool for better morphological appreciation of ganglion cells and thereby helps in establishing the diagnosis of HD.
机译：Hirschsprung疾病（HD）是一种常见的先天性疾病，其特征在于沿着沿着涉及直肠和相邻的近肠道的可变长度的可变长度的肠道神经元的常见障碍。明确的诊断是基于直肠血清血清活组织检查的组织病理学研究。加霉素免疫组化学（IHC）染色增加了诊断产量，因为它可以更好地欣赏成熟和未成熟的神经节细胞，包括缺乏神经节细胞的缺点。评估Calretinin IHC在HD诊断中作为附加技术的有用性常规血红素和曙红染色。材料和方法：这是在2016年8月至2018年7月期间的病理科学，SCB医学院，果实中的病理学系进行了一项前瞻性研究。Fulll厚度直肠活组织检查标本被加工并进行常规苏木辛和曙红染色（H＆amp; e）染色和calretinin ihc染色。彻底寻找神经节细胞在两种类型的染色幻灯片中进行。制表观察结果并比较以诊断准确性。使用统计分析使用社会科学（SPSS）Inc，芝加哥IL版15.0 Windows.Through，Anova和Chi-Square测试进行了统计分析。结果：与常规H＆amp相比，Calretinin IHC提供了卓越的诊断产量。研究了45例的总数。获得了95.4％的敏感度，比55.5％的特异性。它也比H＆amp; e（p = 0.49）。结论：Calretinin是一种优异的免疫组化染色方法，作为可靠的附加诊断工具，用于更好地对神经节细胞的形态欣赏，从而有助于建立HD的诊断。

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