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首页> 外文期刊>CHROMATOGRAPHY >In Situ Photopolymerization of Functionalized Polyacrylamide-Based Preconcentrators for Highly Sensitive Specific Detection of Various Analytes by Microchip Electrophoresis
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In Situ Photopolymerization of Functionalized Polyacrylamide-Based Preconcentrators for Highly Sensitive Specific Detection of Various Analytes by Microchip Electrophoresis

机译：原位基于官能化的聚丙烯酰胺基前浓度的光聚合用于通过微芯片电泳的高敏感性特异性检测各种分析物

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摘要

Microchip electrophoresis (ME) has emerged as a promising tool for rapid analysis of various sample types and it is one of the most important modules of miniaturized total analysis systems. Electrokinetic effects provide both efficient transport and separation of sample components in microfluidic channels. Moreover, miniaturization of devices drastically reduces reagent consumption, significantly decreases analysis time, and allows for easier parallel screening and automation. However, most microfluidic systems are insensitive, which is a serious problem. In order to increase the sensitivity of ME, several on-line pretreatment applications such as specific and non-specific preconcentration, extraction, and derivatization have been proposed. Here, we review various specific on-line preconcentration methods utilizing in situ photopolymerized polyacrylamide-based preconcentrator gels. These techniques allowed for highly sensitive specific detection of various sample types by ME.
机译：Microchip电泳（ME）出现了一种有助于分析各种样品类型的有希望的工具，并且它是小型化总分析系统中最重要的模块之一。 电动效应提供了微流体通道中的有效运输和分离样品组分。 此外，设备的小型化大大降低了试剂消耗，显着降低了分析时间，并且允许更容易平行的筛选和自动化。 然而，大多数微流体系统不敏感，这是一个严重的问题。 为了提高我的灵敏度，已经提出了几种在线预处理应用，例如具体和非特异性的前浓缩，提取和衍生化。 在此，我们在原位光聚合的聚丙烯酰胺基前肠凝胶中审查各种特定的在线预浓缩方法。 这些技术允许我对各种样品类型的高度敏感的特异性检测。

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