Sensitive and Selective LC Determination of 5-Hydroxyindoles Through Online Electrochemical Fluorescence Derivatization Using Benzylamine

Kenichiro TODOROKI; Masaki NAKANO; Hideyuki YOSHIDA; Hitoshi NOHTA; Masatoshi YAMAGUCHI

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Sensitive and Selective LC Determination of 5-Hydroxyindoles Through Online Electrochemical Fluorescence Derivatization Using Benzylamine

机译：使用苄胺通过在线电化学荧光衍生化敏感和选择性LC测定5-羟基吲哚

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In this paper we describe a highly sensitive and selective LC method for the determination of 5-hydroxyindoles (serotonin, N -acetylserotonin, 5-hydroxyindole-3-acetamide, 5-hydroxytryptophan, 5-hydroxyindole-3-acetic acid, and 5-hydroxytryptophol) through post-column electrochemical derivatization and fluorescence detection. These 5-hydroxyindoles can be separated within 40 min by reversed-phase liquid chromatography using 250 mM acetate buffer/acetonitrile [pH 6.5; 95:5 (v/v)] under conditions of isocratic elution; then they were subjected to electrochemical oxidation with benzylamine to produce their corresponding fluorescent derivatives. We detected these derivatives spectrofluorometrically at 480 nm upon excitation at 345 nm. The detection limits (S /N = 3) of these 5-hydroxyindoles were in the range 3.2–180 fmol per 20-μL injection.

机译：在本文中，我们描述了一种高敏感和选择性的LC方法，用于测定5-羟基吲哚（Serotonin， N-乙酰苯甲酰氨基，5-羟基吲哚-3-乙酰胺，5-羟基转孔，5-羟基吲哚-3-乙酸，通过柱柱电化学衍生化和荧光检测，5-羟基特异化。通过反相液相色谱法使用250mM乙酸盐缓冲液/乙腈（pH6.5），可以在40分钟内分离这些5-羟基吲哚。 95：5（v / v）]在等家庭洗脱条件下; 然后用苄胺进行电化学氧化以产生它们相应的荧光衍生物。我们在345nm激发时在480nm下将这些衍生物分光荧光分光荧光分子。这些5-羟基吲哚的检测限（ S / I> n = 3）为每20-μl注射的3.2-180 fmol。 展开▼

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《CHROMATOGRAPHY》 |2021年第1期|共6页

作者
Kenichiro TODOROKI; Masaki NAKANO; Hideyuki YOSHIDA; Hitoshi NOHTA; Masatoshi YAMAGUCHI;
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中图分类化学;

关键词
Serotonin5-HydroxyindoleDerivatizationElectrolytic oxidationFluorescence detectionBenzylamine;

机译：Serotonin5-羟基吲哚胺衍生化电解氧化荧光检测苯并胺;

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Sensitive and Selective LC Determination of 5-Hydroxyindoles Through Online Electrochemical Fluorescence Derivatization Using Benzylamine

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