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Ms3 dominant genetic male sterility for wheat improvement with molecular breeding

机译：MS3主导遗传性雄性不育，用于分子育种的小麦改善

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Genetic dominant male sterility (DMS) has not been widely used as a breeding tool in wheat ( Triticum aestivum L.), although DMS-facilitated backcross, mass selection, half-sib selection, and S 1 family recurrent selection strategies have been described, and Ms2- facilitated recurrent selection has been used in China. Our objective was to revisit these strategies using the tools of molecular breeding. Development of a mechanism for seedling identification of sterile progeny was a key component of designing practical DMS-facilitated molecular breeding systems. The DMS gene Ms3 was previously localized to the centromeric region of chromosome 5A. The centromeric location is an advantage because recombination rates are very low. Once identified, a broadly informative marker would reliably predict the male-sterile phenotype. A set of 429 hybrids incorporating Ms3 were constructed, both within US hard winter wheats, and between these winter wheats and Asian spring wheats. Association of the male-sterile phenotype with those polymorphic DNA sequence tags that localized to chromosome 5A was tested using case-control association analysis. Two highly significant (logarithm of odds [LOD]??30) single nucleotide polymorphism (SNP)–trait associations were obtained. One SNP was developed into a highly sensitive, reliable marker for the Ms3 -associated male-sterile phenotype. Previously described breeding strategies using DMS were updated for trait-targeted marker-assisted backcrossing and gene pyramiding, S 1 recurrent selection, and early-generation genomic selection. Application of DMS to association mapping, with the particular use case of the multiparent advanced generation intercross population, is also described.
机译：遗传优势雄性不育（DMS）未被广泛用作小麦（Triticum Aestivum L）的育种工具，尽管已经描述了DMS促进的回复，质量选择，半SIB选择和S 1家庭经常性选择策略，和MS2促进的经常性选择已在中国使用。我们的目标是使用分子育种的工具重新审视这些策略。一种幼苗鉴定无菌后代的机制是设计实用DMS促进的分子育种系统的关键组分。将DMS基因MS3预先定位于染色体5A的焦化区域。焦化位置是一个优点，因为重组速率非常低。一旦识别，广泛的信息标记将可靠地预测雄性无菌表型。在美国硬冬小麦中以及这些冬小麦和亚洲春小麦之间建造了一组429个杂交物。使用壳体对照关联分析测试雄性无菌表型与那些定位于染色体5a的多晶级序列标签。得到两个非常显着的（对数次数[LOD]？＆Δ30）获得单核苷酸多态性（SNP） - 链接缔合。将一个SNP开发成高度敏感，可靠的标记，用于MS3 - 分配的雄性无菌表型。以前描述了使用DMS使用DMS的育种策略进行特征靶向标记辅助回复和基因金字塔，S 1复发选择以及早期基因组选择。还描述了DMS在关联映射的应用，具有多种前进的多发群体的特定用例。

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    《Crop science》 |2020年第3期|共11页
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    Mary J. Guttieri;

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