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首页> 外文期刊>Egyptian Journal of Medical Human Genetics >Role of interleukins 12B and 17A genetic variation in house dust mites allergy
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Role of interleukins 12B and 17A genetic variation in house dust mites allergy

机译：白细胞介素12B和17A遗传变异在房屋尘螨过敏的作用

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Background:The house dust mites (HDM) constitute a major cause of allergic diseases all over the world. Genes encoding interleukins 12B and 17A which determine the course of T cell-mediated immune response are prime candidates as allergic disease susceptibility. The purpose of this study was to evaluate whether a single-nucleotide polymorphisms (SNP)?of interleukins 12B + 1188A/C (rs3212227) and 17A ?197G/A (rs2275913) confers susceptibility to HDM allergic diseases. Through a case-control study, 120 subjects served as 60 dust mites' allergic patients and 60 healthy non-allergic controls. Total immunoglobulin (Ig) E level, eosinophilic count, serum interleukins 4, 10, 12B, and 17A levels for the studied subjects were measured. Then, genotyping of single-nucleotide polymorphisms (SNPs) at +1188A/C for IL12B and ?197G/A for IL17A gene were conducted using restriction fragment length polymorphisms (RFLP-PCR).ResultsThe present study showed that in HDMs' allergic subjects there was a significant increase in IL12B (+1188 A/C) and IL17A (?197 G/A) genotype variants compared to that of the controls. There was a significant increase in total IgE levels, eosinophil counts, and the levels of both IL-4 and IL-17A, while IL12B was significantly lower in patients compared to that of the controls. There was no significant difference in IL-10 levels between patients and controls.? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ??ConclusionOur findings indicate that IL12B (+1188 A/C) and IL17A (?197G/A) might be associated with an increase in the susceptibility to dust mites’ allergic patients.
机译：背景：房屋尘螨（HDM）构成全世界过敏性疾病的主要原因。编码白细胞介素12b和17a的基因，其确定T细胞介导的免疫应答的过程是作为过敏性疾病易感性的主要候选者。本研究的目的是评估单核苷酸多态性（SNP）吗？白细胞介素12b + 1188a / c（Rs3212227）和17a-197g / a（rs2275913）赋予HDM过敏性疾病的易感性。通过一个案例对照研究，120名受试者服务于60名尘螨过敏患者和60例健康的非过敏反应。测定了研究的总免疫球蛋白（Ig）e水平，嗜酸性粒细胞计数，血清白细胞介素4,10,12b和17a水平。然后，使用限制性片段长度多态性（RFLP-PCR）对IL12B和IL17A基因的单核苷酸多态性（SNPS）的基因分型在IL12B和IL17A基因中进行的。方法研究表明，在HDMS的过敏科目中存在与对照相比，IL12B（+1188A / c）和IL17A（α11188A/ c）和IL17A（α1118A/ a）基因型变体的显着增加。总IgE水平，嗜酸性粒细胞计数和IL-4和IL-17A的水平显着增加，而IL12B与对照的患者显着降低。患者与对照之间的IL-10级别没有显着差异。还还还还还还还还还还还还还还还还还还还还还还还还还还还还还还还还还还还还??记录结果表明，IL12B（+1188A / c）和IL17a（？197g / a）可能与尘螨过敏患者的易感性增加有关。

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