...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of genetics >Cloning and expression of AGL19 gene in two Lonicera macranthoides varieties
【24h】

Cloning and expression of AGL19 gene in two Lonicera macranthoides varieties

机译：两种金发龙桃花生成中AGL19基因的克隆与表达

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

AGLl9 is an important regulator for flowering in plants and critical in controlling the morphogenesis of flower organs. The fulllengthcDNAs of AGL19 in conventional Lonicera macranthoides ( Lm-AGL19 ) and the mutant ‘ Xianglei ’ cultivar ( Lm-XL-AGL19 ) wereobtained using rapid amplification of cDNA ends and the expression vectors for Lm-XL-AGL19 were constructed to investigate the roles of AGL19 in the 'Xianglei' cultivar. Lm-AGL19 (GenBank: MK419948) and Lm-XL-AGL19 (GenBank: MK419948) were isolated from theconventional variety and ' Xianglei ' cultivar of L. macranthoides , respectively. Lm-AGL19 is 1274 bp in length, whereas Lm-XL-AGL19 is 1264-bp long, and both include a 654 bp open reading frame, encoding 217 amino acids, which has a highly conserved MADS_MEF2_likedomain and a moderately conserved K-box domain, belonging to the type II MADS-box family of genes. Quantitative real-time polymerasechain reaction indicated that the expression levels of these genes at different flowering stages were significantly different, and that the geneswere also expressed in stems and leaves. Lm-XL-AGL19 is underexpressed at flowering period 5 that the key time node for corollaexpansion and nonexpansion, while LM-AGL19 is overexpressed during this flowering period. AGL19 was speculated to be a functionalgene causing different phenotypes in the two L. macranthoides varieties. The successfully constructed plant expression vector provides anexperimental reference for further research on the function of this gene and the basis for the excellent phenotype of L. macranthoides ' Xianglei '.
机译：Agll9是一种用于在植物中开花的重要调节因子，在控制花器官的形态发生时至关重要。使用CDNA末端的快速扩增和LM-XL-AGL19的快速扩增，通过CDNA末端（LM-XL-AGL19）和突变体的Xianglei'品种（LM-XL-AGL19）和LM-XL-AGL19的表达载体进行突变体的致植物的Xianglei'品种（LM-XL-AGL19）。研究AGL19在“Xianglei”品种中。 LM-AGL19（Genbank：MK419948）和LM-XL-AGL19（Genbank：MK419948）分别与L. macranthoides的强常数和'Xianglei'品种分离。 LM-AGL19的长度为1274bp，而LM-XL-AGL19为1264-BP长，两者都包括654bp开放阅读框，编码217个氨基酸，具有高度保守的Mads_mef2_likedomain和中度保守的k箱域，属于II型Mads-Box系列基因。定量实时聚合物的反应表明，不同开花阶段的这些基因的表达水平显着不同，并且基因也以茎和叶子表达。 LM-XL-AGL19在开花期5中是缺陷的，CarollaExpASION和NOTEXPANSION的关键时间节点，而LM-AGL19在该开花期间过表达。推测AGL19是官能团，导致两种Macranthoides品种中的不同表型。成功构建的植物表达载体为进一步研究该基因的功能以及L. macranthoides'Xianglei'优异表型的依据提供了一种单纯的参考。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号