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首页> 外文期刊>Journal of genetics >Identification of SNP$_{s}$ in crucial starch biosynthesis genes in rice
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Identification of SNP$_{s}$ in crucial starch biosynthesis genes in rice

机译：鉴定水稻中关键淀粉生物合成基因的SNP $ _ {s} $

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Rice is the foremost crop catering to the major calorific requirement of the human population but has the disadvantage ofhaving high glycaemic index (GI). The fine quality rice varieties, BPT and RNR have been recently identified as having low GI in natureand are grown mostly in southern parts of India. Starch (80%) is the major component of rice endosperm attributing to GI. The study aimedto unravel the molecular basis of low GI through targeted pooled amplicon sequencing of major starch biosynthetic genes. A total of 13candidate genes involved in starch synthesis were amplified and pooled in equimolar proportion for sequencing. Single-nucleotidepolymorphisms (SNP$_{s}$) and insertions/deletions (Indels) were detected in both coding and noncoding regions. Among the genes that areunder study, the highest number of variations were identified in starch synthase I (SSI) followed by starch synthase IIIA (SSIIIA) genes.Nonsynonymous SNP$_{s}$ with high probability of effecting gene function were validated by Sanger sequencing and molecular docking.Identified causative SNP$_{s}$ were mapped on 3000 rice genome database and their allele frequencies were obtained. The outcome of this studyhas a potential to be applied in breeding programmes to obtain low GI rice varieties with added beneficial traits.
机译：米饭是人口主要热量要求的最重要的作物，但具有高血糖指数（GI）的缺点。最近的优质水稻品种，BPT和RNR已被识别为Natureand中的低GI，主要在印度南部地区种植。淀粉（80％）是水稻胚乳归因于GI的主要成分。该研究旨在通过主要淀粉生物合成基因的靶向合并扩增子测序解开低GI的分子基础。扩增淀粉合成中的13个宽容基因，并以等摩尔比例进行排序。在编码和非编码区域中检测到单核肽（SNP $ _ {s} $）和插入/删除（indels）。在研究中的基因中，在淀粉合酶I（SSI）中鉴定了最多的变化，然后鉴定了淀粉合酶IIIa（SSIIIA）基因.Nonsynonymous SNP $ _ {s} $验证了桑佩验证了基因函数的高概率测序和分子对接。识别造成的SNP $ _ {s} $映射到3000个基因组数据库上，并获得了其等位基因频率。该研究的结果是应用在育种计划中的潜力，以获得低GI水稻品种，具有额外的有益特征。

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