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首页> 外文期刊>Journal of Medical Microbiology: An Official Journal of the Pathological Society of Great Britain and Ireland >Detection of SARS-CoV-2 in saliva: implications for specimen transport and storage
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Detection of SARS-CoV-2 in saliva: implications for specimen transport and storage

机译：唾液中SARS-COV-2的检测：标本运输和储存的影响

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Saliva has recently been proposed as a suitable specimen for the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Use of saliva as a diagnostic specimen may present opportunities for SARS-CoV-2 reverse transcription polymerase chain reaction (RT-PCR) testing in remote and low-resource settings. Determining the stability of SARS-CoV-2 RNA in saliva over time is an important step in determining optimal storage and transport times. We undertook an in vitro study to assess whether SARS-CoV-2 could be detected in contrived saliva samples. The contrived saliva samples comprised 10 ml pooled saliva spiked with gamma-irradiated SARS-CoV-2 to achieve a concentration of 2.58×10 ~(4) copies ml SARS-CoV-2, which was subsequently divided into 2 ml aliquots comprising: (i) neat saliva; and a 1?:?1 dilution with (ii) normal saline; (iii) viral transport media, and (iv) liquid Amies medium. Contrived samples were made in quadruplicate, with two samples of each stored at either: (i) room temperature or (ii) 4 °C. SARS-CoV-2 was detected in all SARS-CoV-2 spiked samples at time point 0, day 1, 3 and 7 at both storage temperatures using the N gene RT-PCR assay and time point 0, day 1 and day 7 using the Xpert Xpress SARS-CoV-2 (Cepheid, Sunnyvale, USA) RT-PCR assay. The ability to detect SARS-CoV-2 in saliva over a 1 week period is an important finding that presents further opportunities for saliva testing as a diagnostic specimen for the diagnosis of SARS-CoV-2.
机译：最近已提出唾液作为诊断严重急性呼吸综合征冠状病毒2（SARS-COV-2）的合适标本。使用唾液作为诊断标本可能在远程和低资源设置中呈现SARS-COV-2逆转录聚合酶链反应（RT-PCR）测试的机会。确定唾液中SARS-COV-2 RNA的稳定性随着时间的推移是确定最佳储存和运输时间的重要步骤。我们进行了体外研究，以评估是否可以在有关唾液样本中检测到SARS-COV-2。具有γ辐射的SARS-COV-2的10ml唾液唾液包含10ml合并的唾液，以达到2.58×10〜（4）份拷贝ML SARS-COV-2的浓度，随后将其分成2ml等分试样：（我）整洁的唾液;和1？：1用（ii）稀释（ii）生理盐水; （iii）病毒传输培养基，（iv）液体疗效培养基。以全份数制备了对样品，用两个样品储存在：（I）室温或（II）4℃。在使用N基因RT-PCR测定和时间点0，第1天和第7天在储存温度下的所有SARS-COV-2掺入样品中检测到SARS-COV-2。使用N基因RT-PCR测定和时间点0，第1天和第7天Xpert Xpress SARS-COV-2（Cepheid，Sunnyvale，USA）RT-PCR测定。在1周内检测SARS-COV-2在唾液中的能力是一个重要的发现，这为唾液测试提供了作为诊断SARS-COV-2的诊断标本的进一步的机会。

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