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首页> 外文期刊>Egyptian Journal of Biological Pest Control >Rapid molecular identification of Trichogramma (Hymenoptera: Trichogrammatidae) parasitizing the eggs of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae) in Serbia
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Rapid molecular identification of Trichogramma (Hymenoptera: Trichogrammatidae) parasitizing the eggs of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae) in Serbia

机译：Trichograckmma的快速分子鉴定（Hymenoptera：Trichogrammatidae）寄生欧洲玉米螟，Ostrinia Nubilalis（Lepidoptera：Crambidae）在塞尔维亚的鸡蛋

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摘要

The European corn borer (ECB), Ostrinia nubilalis (Hübner, 1796) (Lepidoptera: Crambidae), is the major pest of maize (Zea mays Linnaeus, 1753) in Serbia. One potential method for managing this pest is the augmentative release of naturally occuring egg parasitoids of the genus Trichogramma. The first step in this process is accurately identifying the naturally occuring species and estimating their natural distribution and abundance. Molecular identification, based on differences in DNA sequences, has commonly been employed for the identification of Trichogramma species. A simple, quick, and accurate molecular assay is urgently required for the identification of two common Trichogramma species, associated with ECB in Serbia: T. brassicae Bezdenko, 1968 and Trichogramma evanescens Westwood, 1833. Such an assay will facilitate an expansive survey of resident populations of Trichogramma associated with ECB across agricultural growing regions of Vojvodina province. A species-specific multiplex PCR assay for the 2 species was developed and validated that assay using a sample of 79 parasitoid wasps reared from ECB egg masses collected from sample sites across Vojvodina province. Trichogramma brassicae was confirmed as the dominant egg parasitoid of ECB in this region, accounting for 77 of the 79 wasps (97.47%). The remaining 2 were confirmed as T. evanescens. Trichogramma brassicae was detected at all 12 sample sites, while T. evanescens was detected at only 2 plots, Mokrin and Nakovo. The species-specific multiplex PCR assay presented herein can provide the basis of a quick, cheap, and reliable means for identifying the species of Trichogramma that parasitize ECB egg masses in Serbia. Two currently documented species, T. brassicae and T. evanescens, are readily diagnosed by the size of the PCR product they produce in the assay. Any additional species are expected to not produce a band of a diagnostic size. Such species would subsequently be identified by sequencing, which may also allow them to be promptly incorporated into a revised assay.
机译：欧洲玉米螟（欧洲央行），汤尼莫罗马利尔（Hübner，1796）（鳞翅目：克拉马芒）是塞尔维亚玉米（Zea Mays Linnaeus，1753）的主要害虫。一种管理这种害虫的潜在方法是天然发生粒子蛋白质的蛋寄生虫的增强释放。该过程的第一步是准确地识别天然存在的物种和估计它们的自然分布和丰度。基于DNA序列的差异的分子鉴定通常用于鉴定略微地图物种。迫切需要一种简单，快速和准确的分子测定，鉴定两种常见的培养基格物种，与塞尔维亚的欧洲核糖菌相关：T. Brassicae Bezdenko，1968年和Trichogramma Evanescens Westwood，1833年。这样的测定将促进对居民的膨胀调查与伏伊迪州省省农业生长地区欧洲央行相关的孕妇群。开发了用于2种的特异性多重PCR测定并验证了使用从vojvodina省上的样品位点收集的ECB蛋群中饲养的79个寄生虫黄蜂的样品进行测定。在该地区的欧洲央行的主要蛋寄生虫证实，略微蛋白质蛋白酶，占79只黄蜂（97.47％）的77例。剩下的2被证实为埃文克斯。在所有12个样品位点检测到Trichograckma Brassicae，而T. EvaneScens仅在仅2个地块，Mokrin和Nakovo中检测到。本文提出的特异性多重PCR测定可以提供快速，廉价，可靠的方法，用于鉴定促使塞尔维亚欧洲欧洲核检生蛋白蛋群的richogramma的种类。通过在测定中产生的PCR产物的大小，有两种目前记录的物种T. Brassicae和T.Vanescens。任何额外的物种都预计不会产生诊断大小的频带。随后通过测序鉴定这些物种，其也可以允许它们迅速纳入修订的测定。

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