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A molecular marker set combining a retrotransposon insertion and SSR polymorphisms is useful for assessing diversity in Vitis

机译：组合转回ransposon插入和SSR多态性的分子标记集可用于评估血管中的多样性

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摘要

Molecular markers, based on DNA polymorphisms, are useful tools for identifying individuals, establishing phylogenetic relationships, managing collections of genetic material or assisting breeding. In the present study, we developed a marker set to differentiate Vitis species, grapevine varieties or clones belonging to the same variety. This novel marker set combines, in four PCR amplifications, the presence/absence of a remarkable retrotransposon, Tvv1 -Δ3460, inserted at its single locus and the SSR polymorphism present within its two LTRs. By studying a collection of Vitaceae accessions, we showed the prevalence of two allelic forms of Tvv1 -Δ3460 - one of which was partially truncated - in Vitis species. Out of the twenty-five studied Vitis species, the insertion of a Tvv1- Δ3460 element was detected in twenty, including Vitis vinifera. The homozygous vs heterozygous state of the element insertion was determined by amplifying the empty site. Additionally, each Tvv1 -Δ3460 LTRs included a microsatellite sequence useful for designing markers based on LTR length. The LTR-SSR markers distinguished most of the fifty-two cultivars and revealed polymorphism within five of the seven varieties studied.
机译：基于DNA多态性的分子标记是用于鉴定个体，建立系统发育关系，管理遗传物质收集或辅助育种的有用工具。在本研究中，我们开发了一种用于区分血管物种，葡萄园品种或属于同一品种的克隆的标记。该新型标记集合在四个PCR扩增中结合，在其单个基因座中插入其单个基因座的呈现/不存在TVV1-Δ3460，并存在于其两个LTR内的SSR多态性。通过研究一系列vitaceae acciach，我们展示了两种等位基因形式的TVv1-Δ3460 - 其中一个是部分截短的 - 在血管炎。在二十五个学习的血管炎物种中，在二十个，包括血管血管膜，包括肺炎菌属的插入。通过扩增空位来测定元素插入的纯合VS杂合状态。此外，每个TVV1-Δ3460LTR包括用于基于LTR长度的设计标记的微卫星序列。 LTR-SSR标记介绍了大部分五十二个品种，并揭示了七种品种中的五个中的五态性。

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