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Differences in responses to Wheat dwarf virus infection in contrasting wheat cultivars Ludwig and Svitava

机译：对比小麦品种Ludwig和Svitava对小麦矮化病毒感染的差异

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The two contrasting cultivars, the very susceptible Ludwig and moderately susceptible Svitava, previously evaluated in field trials to determine their resistance levels to Wheat dwarf virus (WDV), were analysed by four different test methods to allow for the comparison of the methods and to consider whether the resistance level of cv. Svitava can be an effective component of wheat protection from the virus. The differences in the proportion of the diseased plants were observed after the inoculation by the viruliferous leafhoppers. The differences in the cultivar resistance levels were evaluated by comparing the biomass production after the infection. The amount of viral DNA was determined using quantitative polymerase chain reaction (PCR). Concurrently, the cultivar response to the virus infection after the controlled inoculation by the leafhoppers [Psammotettix alienus (Dahlbom, 1850)] under field conditions was further evaluated. The infected plants of cv. Svitava produced five times more dry matter in the vegetative growth stage than cv. Ludwig. The quantitative PCR analysis confirmed the resistance in cv. Svitava. The plant inoculation under a standardised infection pressure showed that the probability of the WDV infection after the inoculation feeding is smaller in the cv. Svitava plants. The comparison of the results from the different test methods shows that the field resistance tests are necessary to obtain a proper assessment of the possibilities for the cultivars’ utilisation in the crop protection.
机译：通过四种不同的试验方法分析了两种对比品种，以前评估了以前在现场试验中评估的耐受对小麦矮化病毒（WDV）的抵抗水平的Svitava，以允许比较方法并考虑比较和考虑无论是CV的电阻水平。 Svitava可以是来自病毒的小麦保护的有效组成部分。在毒叶片接种后观察到患病植物比例的差异。通过比较感染后的生物质生产来评估品种抗性水平的差异。使用定量聚合酶链反应（PCR）测定病毒性DNA的量。同时，进叶蛋白受控接种后对病毒感染的品种反应进一步评估了在现场条件下进行的叶蝉[Psammotettix Alienus（Dahlbom，1850）]。 CV的受感染植物。 Svitava在营养生长阶段的干物质中产生了比CV更多的5倍。 Ludwig。定量PCR分析证实了CV的抗性。 Svitava。在标准化的感染压力下的植物接种表明，在接种饲养后WDV感染的可能性在CV中较小。 Svitava植物。不同试验方法的结果的比较表明，现场电阻测试是必要的，以获得对作物保护中的品种利用可能性的适当评估。

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