• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Journal of automatic chemistry >A Direct and Sensitive Method for Determination of 5-Fluorouracil in Colorectal Cancer Cells: Evaluating the Effect of Stromal Cell on Drug Resistance of Cancer Cells
【24h】

A Direct and Sensitive Method for Determination of 5-Fluorouracil in Colorectal Cancer Cells: Evaluating the Effect of Stromal Cell on Drug Resistance of Cancer Cells

机译：结直肠癌细胞中5-氟尿嘧啶的直接和敏感方法：评价基质细胞对癌细胞耐药性的影响

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Fibroblasts in the stroma play a critical role in tumor evolution. In this study, we assessed the influence of colonic fibroblasts on colon cancer cells treated with 5-fluorouracil (5-FU), and mouse colon cancer cell lines MC38 and colonic fibroblasts NIH3T3 were used in this study. A sensitive and rapid UHPLC-MS/MS method for the quantitation of 5-FU from the cell and their medium has been successfully developed and validated. The cells were lysed with methanol, and the mixture was evaporated and then redissolved to extract intracellular 5-FU. The analysis was performed on UHPLC-MS/MS using an Atlantis T3-C18 column (3? μ m, 2. 1???100?mm) and gradient elution with acetonitrile and 0.1% formic acid in water. Method validation included the following parameters: the matrix effect range 88.82%–93.64% and the recovery range 93.52%–94.56%. The intraday and interday precision and accuracy were <11% and within ±6%, and the stability, specificity, carry-over, dilution effect, and linearity all conformed to the criteria. The method was applied to detect the concentration of 5-FU inside cells and cell culture medium. The preliminary results present that NIH3T3 could enhance the drug resistance of MC38 to 5-FU with a decreased intracellular concentration of 5-FU in MC38, which showed a positive relationship with NIH3T3 number.
机译：基质中的成纤维细胞在肿瘤演变中发挥着关键作用。在这项研究中，我们评估了结肠成纤维细胞对用5-氟尿嘧啶（5-FU）处理的结肠癌细胞的影响，并且在本研究中使用小鼠结肠癌细胞系MC38和结肠成纤维细胞NIH3T3。已成功开发和验证了用于从细胞和其培养基定量5-FU定量的敏感和快速的UHPLC-MS / MS方法。用甲醇裂解细胞，蒸发混合物，然后重新溶解以提取细胞内5-FU。在UHPLC-MS / MS上使用亚特兰蒂斯T3-C18柱（3→μm，2.1→100·100μm）对分析进行，并用乙腈和0.1％甲酸在水中梯度洗脱。方法验证包括以下参数：矩阵效应范围为88.82％-93.64％，恢复范围93.52％-94.56％。中间和白天的精度和准确性<11％，在±6％以内，以及稳定性，特异性，随续，稀释效应和线性均符合标准。应用该方法以检测细胞内5-FU的浓度和细胞培养基。初步结果表明，NIH3T3可以提高MC38至5-FU的耐药性，在MC38中的5-FU细胞内浓度降低，显示与NIH3T3数的阳性关系。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号