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首页> 外文期刊>The Journal of automatic chemistry >Pharmacokinetic Comparisons of Eight Active Components from Raw Farfarae Flos and Honey-Processed Farfarae Flos after Oral Administration in Rats by UHPLC-MS/MS Approaches
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Pharmacokinetic Comparisons of Eight Active Components from Raw Farfarae Flos and Honey-Processed Farfarae Flos after Oral Administration in Rats by UHPLC-MS/MS Approaches

机译：通过UHPLC-MS / MS方法在大鼠口服施用大鼠后八个活性组分的药代动力学比较八种活性成分。

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Farfarae flos (FF) is widely used for cough over thousands of years in China, but little is known about their pharmacokinetics properties. This study was aimed to establish a rapid and accurate ultraperformance liquid chromatography with triple-quadrupole tandem mass spectrometry method for compare pharmacokinetics studies of eight active compounds after oral administration between raw and honey-processed farfarae flos extracts. Optimum separation was performed on a Thermo Hypersil GOLD C18 column (100?mm?×?2.1?mm, 1.9? μ m particles size) with a gradient elution of acetonitrile as mobile phase A and 0.3% formic acid aqueous solution as mobile phase B. The flow rate was set as 0.3?mL/min and separated for 34.0 minutes. Electrospray ionization in the negative ion mode and selected reaction monitoring were used to identify and separate active components. The results met the acceptance criteria and showed that this method exhibited good linear, precision, accuracy, and stability. The extraction recoveries ranged from 81.54% to 104.48%, and the matrix effects ranged from 81.94% to 103.02%. These results show that the validated method could be successfully applied to evaluate the pharmacokinetic study in rats after oral administration of raw farfarae flos (R-FF) and honey-processed farfarae flos (H-FF).
机译：Farfarae Flos（FF）广泛用于中国数千年的咳嗽，但对其药代动力学性质知之甚少。该研究旨在建立一种快速准确的超细型液相色谱，具有三级杆串联质谱法，用于比较原料和蜂蜜加工的Farfarae Flos提取物口服给药后的八个活性化合物的药代动力学研究。在Thermo Hypersil Gold C18柱（100Ωmm≤x≤2.1≤mm，1.9μm，1.9μm，1.9μm，1.9Ω·μm粒子）上进行最佳分离，乙腈作为流动相A和0.3％甲酸水溶液作为流动相B的梯度洗脱。流速设定为0.3？ml / min并分离34.0分钟。在负离子模式中电喷雾电离和选择的反应监测用于鉴定和分离活性组分。结果符合验收标准，并表明该方法表现出良好的线性，精度，精度和稳定性。提取回收率范围为81.54％至104.48％，基质效应范围为81.94％至103.02％。这些结果表明，可以成功地应用验证的方法，以评估原始法氟呋喃（R-FF）和蜂蜜加工的Farfarae Flos（H-FF）后大鼠药代动力学研究。

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