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首页> 外文会议>International Conference on Medical Engineering and Bioinformatics >Pharmacokinetic study of peimine and peiminine in rat plasma after oral administration of Danggui-Beimu-Kushen-Pill by LC-MS/MS
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Pharmacokinetic study of peimine and peiminine in rat plasma after oral administration of Danggui-Beimu-Kushen-Pill by LC-MS/MS

机译：LC-MS / MS在口服施用Danggui-Beimu-Kushen-Pill后大鼠血浆培米嘌呤和Peiminine的药代动力学研究

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摘要

To study the pharmacokinetic parameters of Peimine and Peiminine in rat plasma after oral administration of Danggui-Beimu-Kushen-Pill(DBKP). The analytes were extracted by liquid-liquid extract with diethyl ether and sinomenine was used as internal standard. Chromatographic separation was achieved on a Atlantis T3 column using a gradient elution program with methanol and water containing 10 rnmol/L ammonium acetate. The detection was performed on a triple-quadrupole tandem mass spectrometer by multiple reactions monitoring (MRM) mode in a positive ion mode via electrospray ionization (ESI). Linear calibration curves were obtained in the concentration range of 0.2-25 ng/mL and 0.4-50 ng/mL for peimine and peiminine. Extraction recovery was over 80% in plasma. The intra- and inter-day precision and accuracy were below 10% at three QC levels for both analytes. The method was shown to be effective, convenient, and suitable for simultaneous pharmacokinetic study of peimine and peiminine, which would be beneficial for the clinical use of DBKP.
机译：在口服施用DangGui-Beimu-kushen - 丸（DBKP）后研究大鼠血浆中北菊和北敏的药代动力学参数。通过液 - 液提取物用乙醚萃取分析物，用二乙醚和SINOMENINE作为内标。使用梯度洗脱计划在亚特兰蒂斯T3柱上达到色谱分离，其中含有甲醇和含有10 rnmol / L乙酸铵的水。通过电喷雾电离（ESI）在正离子模式下通过多重反应监测（MRM）模式对三重rupRupole串联质谱仪进行检测。在培米菊和PeImininine的浓度范围内获得线性校准曲线，为0.2-25ng / ml和0.4-50ng / ml。血浆中提取恢复超过80％。对于两种分析物，在三个QC水平下，血液内和日间的精度和精度低于10％。该方法显示有效，方便，适用于北菊和北美尼尼尼尼尼尼尼尼莫的同时药代动力学研究，这对DBKP的临床使用是有益的。

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